BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4263

Title: dNumb PTB Domain Complexed with a Phosphotyrosine Peptide, NMR, Ensemble of Structures.

Authors: Zwahlen, C.; Li, S.; Vincent, S.; Mcglade, C.; Kay, L.; Pawson, T.; Forman-Kay, J.

Citation: Zwahlen, C.; Li, S.; Vincent, S.; Mcglade, C.; Kay, L.; Pawson, T.; Forman-Kay, J.. "Structure of a Numb PTB Domain-peptide Complex: A Basis for Diverse Binding Specificity"  Nat. Struct. Biol. 5, 1075-1083 (1998).

Assembly members:
PTB domain of Drosophila Numb protein, polymer, 160 residues, Formula weight is not available
GPpY peptide, polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: Fruit Fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PTB domain of Drosophila Numb protein: GSPGIPDRVPESSKPHQWQA DEEAVRSATCSFSVKYLGCV EVFESRGMQVCEEALKVLRQ SRRRPVRGLLHVSGDGLRVV DDETKGLIVDQTIEKVSFCA PDRNHERGFSYICRDGTTRR WMCHGFLACKDSGERLSHAV GCAFAVCLERKQRRTRAAAS
GPpY peptide: AYIGPXL

Data typeCount
13C chemical shifts638
15N chemical shifts171
1H chemical shifts916

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dNumb_PTB_one1
2GPpY_peptide_one2
3dNumb_PTB_two1
4GPpY_peptide_two2

Entities:

Entity 1, dNumb_PTB_one 160 residues - Formula weight is not available

1   GLYSERPROGLYILEPROASPARGVALPRO
2   GLUSERSERLYSPROHISGLNTRPGLNALA
3   ASPGLUGLUALAVALARGSERALATHRCYS
4   SERPHESERVALLYSTYRLEUGLYCYSVAL
5   GLUVALPHEGLUSERARGGLYMETGLNVAL
6   CYSGLUGLUALALEULYSVALLEUARGGLN
7   SERARGARGARGPROVALARGGLYLEULEU
8   HISVALSERGLYASPGLYLEUARGVALVAL
9   ASPASPGLUTHRLYSGLYLEUILEVALASP
10   GLNTHRILEGLULYSVALSERPHECYSALA
11   PROASPARGASNHISGLUARGGLYPHESER
12   TYRILECYSARGASPGLYTHRTHRARGARG
13   TRPMETCYSHISGLYPHELEUALACYSLYS
14   ASPSERGLYGLUARGLEUSERHISALAVAL
15   GLYCYSALAPHEALAVALCYSLEUGLUARG
16   LYSGLNARGARGTHRARGALAALAALASER

Entity 2, GPpY_peptide_one 7 residues - Formula weight is not available

1   ALATYRILEGLYPROPTRLEU

Samples:

sample_1: PTB domain of Drosophila Numb protein, [U-100% 13C; U-100% 15N], 1.2 mM; GPpY peptide, [99% 12C; 99% 14N]-pY, ALA [100% 13C, 100% 15N]-TYR, ILE, GLY, PRO, LEU ,

sample_conditions_1: ionic strength: 0.22; pH: 6.0; pressure: 1 bar; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablesample_conditions_1

Software:

NMRPipe - NMR data processing

NMRView - Data analysis, Peak picking, Semi-automated assignment

X-PLOR v3.158 - Structure calculation

ARIA - Structure calculation

NMR spectrometers:

  • Varian Unity Plus 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

BMRB 4683
PDB
GB AAA28730 AAF52776 AAK93152 AAN10693 ACL68752
REF NP_001260291 NP_523523 NP_723460 XP_001357245 XP_001964877
SP P16554