BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4265

Title: 1H, 15N and 13C Resonance Assignments for the 22KDa LC1 Light Chain from Chlamydomonas Outer Arm Dynein

Authors: Wu, Hongwei; Maciejewski, Mark; Benashski, Sharon; Mullen, Gregory; King, Stephen

Citation: Wu, Hongwei; Maciejewski, Mark; Benashski, Sharon; Mullen, Gregory; King, Stephen. "1H, 15N and 13C Resonance Assignments for the 22KDa LC1 Light Chain from Chlamydomonas Outer Arm Dynein "  to be published ., .-. (1999).

Assembly members:
Dynein light chain 1, polymer, 199 residues, Formula weight is not available

Natural source:   Common Name: Chlamydomonas reinhardtii   Taxonomy ID: 3055   Superkingdom: Eucaryote   Kingdom: not available   Genus/species: Chlamydomonas reinhardtii

Experimental source:   Production method: recombinant_technology

Entity Sequences (FASTA):
Dynein light chain 1: HMAKATTIKDAIRIFEERKS VVATEAEKVELHGMIPPIEK MDATLSTLKACKHLALSTNN IEKISSLSGMENLRILSLGR NLIKKIENLDAVADTLEELW ISYNQIASLSGIEKLVNLRV LYMSNNKITNWGEIDKLAAL DKLEDLLLAGNPLYNDYKEN NATSEYRIEVVKRLPNLKKL DGMPVDVDEREQANVARGG

Data sets:
Data typeCount
1H chemical shifts1088
13C chemical shifts709
15N chemical shifts209

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC11

Entities:

Entity 1, LC1 199 residues - Formula weight is not available

1   HISMETALALYSALATHRTHRILELYSASP
2   ALAILEARGILEPHEGLUGLUARGLYSSER
3   VALVALALATHRGLUALAGLULYSVALGLU
4   LEUHISGLYMETILEPROPROILEGLULYS
5   METASPALATHRLEUSERTHRLEULYSALA
6   CYSLYSHISLEUALALEUSERTHRASNASN
7   ILEGLULYSILESERSERLEUSERGLYMET
8   GLUASNLEUARGILELEUSERLEUGLYARG
9   ASNLEUILELYSLYSILEGLUASNLEUASP
10   ALAVALALAASPTHRLEUGLUGLULEUTRP
11   ILESERTYRASNGLNILEALASERLEUSER
12   GLYILEGLULYSLEUVALASNLEUARGVAL
13   LEUTYRMETSERASNASNLYSILETHRASN
14   TRPGLYGLUILEASPLYSLEUALAALALEU
15   ASPLYSLEUGLUASPLEULEULEUALAGLY
16   ASNPROLEUTYRASNASPTYRLYSGLUASN
17   ASNALATHRSERGLUTYRARGILEGLUVAL
18   VALLYSARGLEUPROASNLEULYSLYSLEU
19   ASPGLYMETPROVALASPVALASPGLUARG
20   GLUGLNALAASNVALALAARGGLYGLY

Samples:

sample_one: Dynein light chain 1, [U-15N], 1.0 mM

sample_two: Dynein light chain 1, [U-13C; U-15N], 1.0 mM

sample_conditions_one: pH: 6.76 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_onenot availablenot available
HN(CO)CAsample_onenot availablenot available
HNCACBsample_onenot availablenot available
CBCA(CO)NHsample_onenot availablenot available
HNCOsample_onenot availablenot available
HNHAsample_onenot availablenot available
(HB)CBCACO(CA)HAsample_onenot availablenot available
1H-15N_NOESY-HSQCsample_onenot availablenot available
1H-15N_TOCSY-HSQCsample_onenot availablenot available
C(CO)NH-TOCSYsample_onenot availablenot available
HC(CO)NH-TOCSYsample_onenot availablenot available
HCCH-TOCSYsample_onenot availablenot available
1H-15N_HSQCsample_onenot availablenot available
1H-13C_HSQCsample_onenot availablenot available

Software:

Felix v95 - data processing

Xeasy v1.3.13 - assignments

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
GB AAD41040 EDP06769
REF XP_001701794
SP Q9XHH2