BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4268

Title: 7-Fe Ferredoxin from Bacillus Schlegelii

Authors: Aono, S.; Bentrop, D.; Bertini, I.; Donaire, A.; Luchinat, C.; Niikura, Y.; Rosato, A.

Citation: Aono, S.; Bentrop, D.; Bertini, I.; Donaire, A.; Luchinat, C.; Niikura, Y.; Rosato, A.. "Solution Structure of the Oxidized Fe7S8 Ferredoxin from the Thermophilic Bacterium Bacillus Schlegelii by 1H NMR Spectroscopy"  Biochemistry 37, 9812-9826 (1998).

Assembly members:
7-Fe Ferredoxin, polymer, 77 residues, Formula weight is not available
FS3, non-polymer, 295.795 Da.
FS4, non-polymer, 351.640 Da.

Natural source:   Common Name: B. schlegelli   Taxonomy ID: not available   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus schlegelii bacilli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
7-Fe Ferredoxin: AYVITEPCIGTKDASCVEVC PVDCIHEGEDQYYIDPDVCI DCGACEAVCPVSAIYHEDFV PEEWKSYIQKNRDFFKK

Data sets:
Data typeCount
1H chemical shifts407

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fd peptide1
2FS32
3FS43

Entities:

Entity 1, Fd peptide 77 residues - Formula weight is not available

1   ALATYRVALILETHRGLUPROCYSILEGLY
2   THRLYSASPALASERCYSVALGLUVALCYS
3   PROVALASPCYSILEHISGLUGLYGLUASP
4   GLNTYRTYRILEASPPROASPVALCYSILE
5   ASPCYSGLYALACYSGLUALAVALCYSPRO
6   VALSERALAILETYRHISGLUASPPHEVAL
7   PROGLUGLUTRPLYSSERTYRILEGLNLYS
8   ASNARGASPPHEPHELYSLYS

Entity 2, FS3 - Fe3 S4 - 295.795 Da.

1   FS3

Entity 3, FS4 - Fe4 S4 - 351.640 Da.

1   FS4

Samples:

sample_one: 7-Fe Ferredoxin2 – 3.5 mM

sample_conditions: ionic strength: 0.020 M; pH: 6.5 na; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions
TOCSYsample_onenot availablesample_conditions
DQF-COSYsample_onenot availablesample_conditions
1D-NOEsample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4229 4231
PDB
DBJ BAA06187
SP Q45560