BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4269

Title: 1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase

Authors: Pan, Borlan; Mullen, Greg

Citation: Pan, Borlan; Mullen, Greg. "1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase"  J. Biomol. NMR 13, 307-308 (1999).

Assembly members:
resolvase, polymer, 105 residues, 11608 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
resolvase: MRLFGYARVSTSQQSLDIQV RALKDAGVKANRIFTDKASG SSSDRKGLDLLRMKVEEGDV ILVKKLDRLGRDTADMIQLI KEFDAQGVSIRFIDDGISTD GEMGK

Data sets:
Data typeCount
1H chemical shifts696
13C chemical shifts411
15N chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1resolvase1

Entities:

Entity 1, resolvase 105 residues - 11608 Da.

1   METARGLEUPHEGLYTYRALAARGVALSER
2   THRSERGLNGLNSERLEUASPILEGLNVAL
3   ARGALALEULYSASPALAGLYVALLYSALA
4   ASNARGILEPHETHRASPLYSALASERGLY
5   SERSERSERASPARGLYSGLYLEUASPLEU
6   LEUARGMETLYSVALGLUGLUGLYASPVAL
7   ILELEUVALLYSLYSLEUASPARGLEUGLY
8   ARGASPTHRALAASPMETILEGLNLEUILE
9   LYSGLUPHEASPALAGLNGLYVALSERILE
10   ARGPHEILEASPASPGLYILESERTHRASP
11   GLYGLUMETGLYLYS

Samples:

sample_one: resolvase, [U-15N], 2.0 mM; D2O 10%; H2O 90%

sample_two: resolvase, [U-13C; U-15N], 2.0 mM

sample_conditions_one: pH: 6.5; temperature: 298 K; ionic strength: 1.0

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablesample_conditions_one
HNCOCAnot availablenot availablesample_conditions_one
HNCACBnot availablenot availablesample_conditions_one
CBCACONHnot availablenot availablesample_conditions_one
HNCO,not availablenot availablesample_conditions_one
CCONHnot availablenot availablesample_conditions_one
HCCONHnot availablenot availablesample_conditions_one
HCCH-TOCSYnot availablenot availablesample_conditions_one
1H-NOESYnot availablenot availablesample_conditions_one

Software:

FELIX v95 - Data Processing

XEASY v1.3.13 - Spectral visualization and resonance assignment

NMR spectrometers:

  • Varian . 500 MHz
  • Varian . 600 MHz

Related Database Links:

PDB
DBJ BAA03915 BAA97879
EMBL CAA42759 CTC93933 CUH58641
GB AAZ05410 ABX47149 ABX47153 ACB02468 ACT33552
PRF 0812253A
REF NP_061388 WP_001235704 WP_001749204 WP_005766999 WP_010129789
SP P03012