BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4271

Title: Sequence Specific 1H, 13C and 15N Assignments of a Calcium Binding Protein from Entamoeba Histolytica

Authors: Govil, Girjesh; Chary, Kandala; Sahu, Sarata; Chauhan, Sanjay; Bhattacharya, A.; Atreya, H.

Citation: Govil, Girjesh; Chary, Kandala; Sahu, Sarata; Chauhan, Sanjay; Bhattacharya, A.; Atreya, H.. "Sequence Specific 1H, 13C and 15N Assignments of a Calcium Binding Protein from Entamoeba Histolytica"  J. Biomol. NMR 14, 93-94 (1999).

Assembly members:
Entamoeba Histolytica CaBP, polymer, 134 residues, 14939 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Entamoeba Histolytica   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba histolytica

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Entamoeba Histolytica CaBP: MAEALFKEIDVNGDGAVSYE EVKAFVSKKRAIKNEQLLQL IFKSIDADGNGEIDQNEFAK FYGSIQGQDLSDDKIGLKVL YKLMDVDGDGKLTKEEVTSF FKKHGIEKVAEQVMKADANG DGYITLEEFLEFSL

Data sets:
Data typeCount
1H chemical shifts496
13C chemical shifts380
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Eh CaBP1
2Calcium Ion2

Entities:

Entity 1, Eh CaBP 134 residues - 14939 Da.

1   METALAGLUALALEUPHELYSGLUILEASP
2   VALASNGLYASPGLYALAVALSERTYRGLU
3   GLUVALLYSALAPHEVALSERLYSLYSARG
4   ALAILELYSASNGLUGLNLEULEUGLNLEU
5   ILEPHELYSSERILEASPALAASPGLYASN
6   GLYGLUILEASPGLNASNGLUPHEALALYS
7   PHETYRGLYSERILEGLNGLYGLNASPLEU
8   SERASPASPLYSILEGLYLEULYSVALLEU
9   TYRLYSLEUMETASPVALASPGLYASPGLY
10   LYSLEUTHRLYSGLUGLUVALTHRSERPHE
11   PHELYSLYSHISGLYILEGLULYSVALALA
12   GLUGLNVALMETLYSALAASPALAASNGLY
13   ASPGLYTYRILETHRLEUGLUGLUPHELEU
14   GLUPHESERLEU

Entity 2, Calcium Ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Entamoeba Histolytica CaBP, [U-98% 13C; U-98% 15N], 3 – 4 mM

sample_2: Entamoeba Histolytica CaBP, [U-98% 15N], 3 – 4 mM

cabp1: pH: 6.0; temperature: 308 K; ionic strength: 0.05 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablecabp1
15N-TOCSY-HSQCnot availablenot availablecabp1
15N-NOESY-HSQCnot availablenot availablecabp1
HCAnot availablenot availablecabp1
H(CO)CAnot availablenot availablecabp1
HCOnot availablenot availablecabp1
CBCANHnot availablenot availablecabp1
CBCA(CO)NHnot availablenot availablecabp1

Software:

Felix v95.0 - Some C programmes were written with peak lists from triple resonance spectra as input to make the assignments semi-automated.

NMR spectrometers:

  • Varian Unity plus 600 MHz

Related Database Links:

BMRB 19197
PDB
DBJ BAN39246
GB AAA29089 EAL48959 EKE39141 EMD43507 EMH75928
REF XP_008858522 XP_654345
SP P38505