BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4276

Title: Solution Structure and Dynamics of the Plasminogen Kringle 2-AMCHA Complex: 3/1-Helix in Homologous Domains   PubMed: 10625440

Authors: Marti, D.; Schaller, J.; Llinas, M.

Citation: Marti, D.; Schaller, J.; Llinas, M.. "Solution structure and dynamics of the plasminogen kringle 2-AMCHA complex: 3(1)-helix in homologous domains"  Biochemistry 38, 15741-15755 (1999).

Assembly members:
Plasminogen, polymer, 83 residues, 9631 Da.
AMH, non-polymer, 157.210 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: coli

Entity Sequences (FASTA):
Plasminogen: TSEECMHGSGENYDGKISKT MSGLECQAWDSQSPHAHGYI PSKFPNKNLKKNYCRNPDRE LRPWCFTTDPNKRWELCDIP RCT

Data sets:
Data typeCount
13C chemical shifts81
15N chemical shifts89
1H chemical shifts558
coupling constants67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pgn1
2TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID2

Entities:

Entity 1, Pgn 83 residues - 9631 Da.

1   THRSERGLUGLUCYSMETHISGLYSERGLY
2   GLUASNTYRASPGLYLYSILESERLYSTHR
3   METSERGLYLEUGLUCYSGLNALATRPASP
4   SERGLNSERPROHISALAHISGLYTYRILE
5   PROSERLYSPHEPROASNLYSASNLEULYS
6   LYSASNTYRCYSARGASNPROASPARGGLU
7   LEUARGPROTRPCYSPHETHRTHRASPPRO
8   ASNLYSARGTRPGLULEUCYSASPILEPRO
9   ARGCYSTHR

Entity 2, TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID - C8 H15 N O2 - 157.210 Da.

1   AMH

Samples:

sample_1: Plasminogen 2.7 mM; TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID 8.1 mM

sample_2: Plasminogen, [U-15N], 2.7 mM; TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID 8.1 mM

sample_3: Plasminogen, [U-15N; U-13C], 1.2 mM; TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID 3.6 mM

sample_cond_1: pH: 5.1; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
COSYnot availablenot availablesample_cond_1
NOESYnot availablenot availablesample_cond_1
TOCSYnot availablenot availablesample_cond_1
3D-NOESY HSQCnot availablenot availablesample_cond_1
3D-TOCSY HSQCnot availablenot availablesample_cond_1
HNCAnot availablenot availablesample_cond_1
HN(CO)CAnot availablenot availablesample_cond_1
HCC(CO)NHnot availablenot availablesample_cond_1
3D HNHAnot availablenot availablesample_cond_1

Software:

FELIX v95.0 -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB