BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4281

Title: Backbone and Side Chain 1H, 13C, and 15N Chemical Shift Assignments for AbrBN

Authors: Bobay, Benjamin; Andreeva, Antonina; Mueller, Geoffrey; Cavanagh, John; Murzin, Alexey

Citation: Bobay, Benjamin; Andreeva, Antonina; Mueller, Geoffrey; Cavanagh, John; Murzin, Alexey. "Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins"  . ., .-..

Assembly members:
AbrB, polymer, 53 residues, 6100 Da.

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AbrB: MKSTGIVRKVDELGRVVIPI ELRRTLGIAEKDALEIYVDD EKIILKKYKPNMT

Data sets:
Data typeCount
1H chemical shifts403
13C chemical shifts238
15N chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AbrBN subunit 11
2AbrBN subunit 21

Entities:

Entity 1, AbrBN subunit 1 53 residues - 6100 Da.

1   METLYSSERTHRGLYILEVALARGLYSVAL
2   ASPGLULEUGLYARGVALVALILEPROILE
3   GLULEUARGARGTHRLEUGLYILEALAGLU
4   LYSASPALALEUGLUILETYRVALASPASP
5   GLULYSILEILELEULYSLYSTYRLYSPRO
6   ASNMETTHR

Samples:

sample_one: AbrB, [U-99% 13C; U-99% 15N], 1.5 – 2.5 mM; KCl 15 mM; K-PO 10 mM; DTT 1 mM; NaN3 0.02%; D20 5%; H2O 95%

sample_two: AbrB, [U-99% 13C; U-99% 15N], 1.5 – 2.5 mM; KCl 15 mM; K-PO 10 mM; DTT 1 mM; NaN3 0.02%; D20 5%; H2O 95%

standard_h2o_conditions: pH: 5.8; temperature: 305 K

standard_d2o_conditions: pH*: 5.8; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY (120mS and 150mS mixing time)not availablenot availablenot available
1H-13C COESY (120mS and 120mS folded mixing time)not availablenot availablenot available
3-D HNCAnot availablenot availablenot available
3-D HN(CO)CAnot availablenot availablenot available
3-D HNCOnot availablenot availablenot available
3-D HN(CA)COnot availablenot availablenot available
3-D CBCA(CO)NHnot availablenot availablenot available
3-D HNCACBnot availablenot availablenot available
D2O exchange via 2-D 1H-15N HSQCnot availablenot availablenot available
CC-TOCSY-(CO)NHnot availablenot availablenot available
H(CC-TOCSY-CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

ARIA v1.2 -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17650
PDB
DBJ BAA05272 BAI83483 BAL15827 BAM48968 BAM56238
EMBL CAA31307 CAA43955 CAB11813 CBI41163 CCF03572
GB AAA22195 AAP07140 AAP24090 AAS38971 AAT29114
PRF 1715209A
REF NP_387918 NP_829939 NP_842604 WP_000843034 WP_000843035
SP P08874