BMRB Entry 4286
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4286
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Citation: Fiebig, Klaus; Rice, Luke; Pollock, Elizabeth; Brunger, Axel. "Folding Intermediates of SNARE Complex Assembly" Nat. Struct. Biol. 6, 117-123 (1999).
Assembly members:
Snc1, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Snc1: GSPEFMSSSTPFDPYALSEH
DEERPQNVQSKSRTAELQAE
IDDTVGIMRDNINKVAERGE
RLTSIEDKADNLAVSAQGFK
RGANRVRKAMWYKDLKMK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 512 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Snc1 | 1 |
Entities:
Entity 1, Snc1 98 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLU | PHE | MET | SER | SER | SER | THR | ||||
| 2 | PRO | PHE | ASP | PRO | TYR | ALA | LEU | SER | GLU | HIS | ||||
| 3 | ASP | GLU | GLU | ARG | PRO | GLN | ASN | VAL | GLN | SER | ||||
| 4 | LYS | SER | ARG | THR | ALA | GLU | LEU | GLN | ALA | GLU | ||||
| 5 | ILE | ASP | ASP | THR | VAL | GLY | ILE | MET | ARG | ASP | ||||
| 6 | ASN | ILE | ASN | LYS | VAL | ALA | GLU | ARG | GLY | GLU | ||||
| 7 | ARG | LEU | THR | SER | ILE | GLU | ASP | LYS | ALA | ASP | ||||
| 8 | ASN | LEU | ALA | VAL | SER | ALA | GLN | GLY | PHE | LYS | ||||
| 9 | ARG | GLY | ALA | ASN | ARG | VAL | ARG | LYS | ALA | MET | ||||
| 10 | TRP | TYR | LYS | ASP | LEU | LYS | MET | LYS |
Samples:
sample_one: Snc1, [U-99% 15N], 1.2 mM
sample_conditions: ionic strength: 220; pH: 4.5; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N-HSQC | sample_one | not available | sample_conditions |
| 1H-15N-NOESY-HSQC | sample_one | not available | sample_conditions |
| 1H-15N-TOCSY-HSQC | sample_one | not available | sample_conditions |
Software:
FELIX v95 - transformation
XEasy v1.3.11 - Assignment
NMR spectrometers:
- Varian . 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA21395 |
| EMBL | CAY77620 |
| GB | AAA35069 AAC05002 AHY74337 AJO92283 AJO92361 |
| REF | NP_009372 |
| SP | P31109 |
| TPG | DAA06958 |
| AlphaFold | P31109 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks