BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4299

Title: 1H, 13C and 15N Resonance Assignments of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin Pyrophosphokinase and its Complex with MgAMPPCP

Authors: Shi, Genbin; Gao, Jinhai; Yan, Honggao

Citation: Shi, Genbin; Gao, Jinhai; Yan, Honggao. "1H, 13C and 15N Resonance Assignments of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin Pyrophosphokinase and its Complex with MgAMPPCP"  J. Biomol. NMR 14, 189-190 (1999).

Assembly members:
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, polymer, 158 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: TVAYIAIGSNLASPLEQVNA ALKALGDIPESHILTVSSFY RTPPLGPQDQPDYLNAAVAL ETSLAPEELLNHTQRIELQQ GRVRKAERWGPRTLDLDIML FGNEVINTERLTVPHYDMKN RGFMLWPLFEIAPELVFPDG EMLRQILHTRAFDKLNKW

Data sets:
Data typeCount
13C chemical shifts240
15N chemical shifts127
1H chemical shifts858

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HPPK_monomer1

Entities:

Entity 1, HPPK_monomer 158 residues - Formula weight is not available

1   THRVALALATYRILEALAILEGLYSERASN
2   LEUALASERPROLEUGLUGLNVALASNALA
3   ALALEULYSALALEUGLYASPILEPROGLU
4   SERHISILELEUTHRVALSERSERPHETYR
5   ARGTHRPROPROLEUGLYPROGLNASPGLN
6   PROASPTYRLEUASNALAALAVALALALEU
7   GLUTHRSERLEUALAPROGLUGLULEULEU
8   ASNHISTHRGLNARGILEGLULEUGLNGLN
9   GLYARGVALARGLYSALAGLUARGTRPGLY
10   PROARGTHRLEUASPLEUASPILEMETLEU
11   PHEGLYASNGLUVALILEASNTHRGLUARG
12   LEUTHRVALPROHISTYRASPMETLYSASN
13   ARGGLYPHEMETLEUTRPPROLEUPHEGLU
14   ILEALAPROGLULEUVALPHEPROASPGLY
15   GLUMETLEUARGGLNILELEUHISTHRARG
16   ALAPHEASPLYSLEUASNLYSTRP

Samples:

sample_one: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, [U-15N], 1.5 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_two: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, [U-15N;U-13C], 1.5 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4300
PDB
DBJ BAB96719 BAJ41992 BAL37452
EMBL CBI99639 CCP97244 CDJ70723 CDK83585 CDY54783
GB AAB53446 AAC73253 ACA79131 ACB01321 ACR61761
REF NP_414684 WP_000215127 WP_000215131 WP_000215137 WP_000215138
SP P26281