BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4309

Title: Backbone NMR Assignment and Secondary Structure of the 19 kDa Hemophore HasA

Authors: Izadi-Pruneyre, Nadia; Wolff, Nicolas; Castagne, Claire; Czisch, Michael; Wandersman, Cecile; Delepierre, Muriel; Lecroisey, Anne

Citation: Izadi-Pruneyre, Nadia; Wolff, Nicolas; Castagne, Claire; Czisch, Michael; Wandersman, Cecile; Delepierre, Muriel; Lecroisey, Anne. "Backbone NMR Assignment and Secondary Structure of the 19 kDa Hemophore HasA"  J. Biomol. NMR 14, 193-194 (1999).

Assembly members:
HasA, polymer, 178 residues, 18284.7 Da.

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 615   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Serratia marcescens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HasA: AFSVNYDSSFGGYSIHDYLG QWASTFGDVNHTNGNVTDAN SGGFYGGSLSGSQYAISSTA NQVTAFVAGGNLTYTLFNEP AHTLYGQLDSLSFGDGLSGG DTSPYSIQVPDVSFGGLNLS SLQAQGHDGVVHQVVYGLMS GDTGALETALNGILDDYGLS VNSTFDQVAAATAVGVQH

Data sets:
Data typeCount
13C chemical shifts337
15N chemical shifts172
1H chemical shifts369

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HasA1

Entities:

Entity 1, HasA 178 residues - 18284.7 Da.

1   ALAPHESERVALASNTYRASPSERSERPHE
2   GLYGLYTYRSERILEHISASPTYRLEUGLY
3   GLNTRPALASERTHRPHEGLYASPVALASN
4   HISTHRASNGLYASNVALTHRASPALAASN
5   SERGLYGLYPHETYRGLYGLYSERLEUSER
6   GLYSERGLNTYRALAILESERSERTHRALA
7   ASNGLNVALTHRALAPHEVALALAGLYGLY
8   ASNLEUTHRTYRTHRLEUPHEASNGLUPRO
9   ALAHISTHRLEUTYRGLYGLNLEUASPSER
10   LEUSERPHEGLYASPGLYLEUSERGLYGLY
11   ASPTHRSERPROTYRSERILEGLNVALPRO
12   ASPVALSERPHEGLYGLYLEUASNLEUSER
13   SERLEUGLNALAGLNGLYHISASPGLYVAL
14   VALHISGLNVALVALTYRGLYLEUMETSER
15   GLYASPTHRGLYALALEUGLUTHRALALEU
16   ASNGLYILELEUASPASPTYRGLYLEUSER
17   VALASNSERTHRPHEASPGLNVALALAALA
18   ALATHRALAVALGLYVALGLNHIS

Samples:

sample_1: HasA, [U-95% 15N], 2.0 mM

sample_2: HasA, [15N 95%]-Leu, 2.0 mM

sample_3: HasA, [U-95% 13C; U-95% 15N], 2.0 mM

Ex-condition: pH: 5.6 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEx-condition
1H-15N TOCSY-HSQCnot availablenot availableEx-condition
1H-15N NOESY-HSQCnot availablenot availableEx-condition
HNCACBnot availablenot availableEx-condition
CBCA(CO)NHnot availablenot availableEx-condition
HNCOnot availablenot availableEx-condition
1H-15N HMQC-Jnot availablenot availableEx-condition

Software:

XEASY v1.2 -

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
EMBL CAA57068
GB KFD10589 KFL05309
REF WP_016928898
SP Q54450