BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4312

Title: 1H, 15N and 13C Resonance Assignments for the Bromodomain of the Histone Acetyltransferase hsP/CAF

Authors: Dhalluin, Christophe; Carlson, Justin; Zeng, Lei; He, Cheng; Aggarwal, Aneel; Zhou, Ming-Ming

Citation: Dhalluin, Christophe; Carlson, Justin; Zeng, Lei; He, Cheng; Aggarwal, Aneel; Zhou, Ming-Ming. "1H, 15N and 13C Resonance Assignments for the Bromodomain of the Histone Acetyltransferase hsP/CAF"  J. Biomol. NMR ., .-..

Assembly members:
histone acetyltransferase bromodomain, polymer, 118 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
histone acetyltransferase bromodomain: GSHMSKEPRDPDQLYSTLKS ILQQVKSHQSAWPFMEPVKR TEAPGYYEVIRSPMDLKTMS ERLKNRYYVSKKLFMADLQR VFTNCKEYNAPESEYYKCAN ILEKFFFSKIKEAGLIDK

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts107
1H chemical shifts708

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1brd subunit 11

Entities:

Entity 1, brd subunit 1 118 residues - Formula weight is not available

1   GLYSERHISMETSERLYSGLUPROARGASP
2   PROASPGLNLEUTYRSERTHRLEULYSSER
3   ILELEUGLNGLNVALLYSSERHISGLNSER
4   ALATRPPROPHEMETGLUPROVALLYSARG
5   THRGLUALAPROGLYTYRTYRGLUVALILE
6   ARGSERPROMETASPLEULYSTHRMETSER
7   GLUARGLEULYSASNARGTYRTYRVALSER
8   LYSLYSLEUPHEMETALAASPLEUGLNARG
9   VALPHETHRASNCYSLYSGLUTYRASNALA
10   PROGLUSERGLUTYRTYRLYSCYSALAASN
11   ILELEUGLULYSPHEPHEPHESERLYSILE
12   LYSGLUALAGLYLEUILEASPLYS

Samples:

sample_1: histone acetyltransferase bromodomain, [U-13C; U-15N; U-75% 2H], 1.0 mM; phosphate buffer 100 mM; perdeuterated DTT 5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: histone acetyltransferase bromodomain, [U-13C; U-15N], 1.0 mM; phosphate buffer 100 mM; perdeuterated DTT 5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

Ex-cond_1: pH: 6.5 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availableEx-cond_1
2D 1H-13C HSQCnot availablenot availableEx-cond_1
3D HNCACBnot availablenot availableEx-cond_1
3D HN(CO)CACBnot availablenot availableEx-cond_1
3D (H)C(CO)NH-TOCSYnot availablenot availableEx-cond_1
3D HNHAnot availablenot availableEx-cond_1
3D HCCH TOCSYnot availablenot availableEx-cond_1
15N edited TOCSYnot availablenot availableEx-cond_1
15N edited NOESYnot availablenot availableEx-cond_1
13C edited NOESYnot availablenot availableEx-cond_1

Software:

NMRPipe v3.1 - spectra Processing

NMRVIEW v3.1 - spectra analysis

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

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