BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4373

Title: 1H, 15N, and 13C Resonance Assignment of the PH Domain from C. elegans UNC-89

Authors: Blomberg, Niklas; Sattler, Michael; Nilges, Michael

Citation: Blomberg, Niklas; Sattler, Michael; Nilges, Michael. "1H, 15N, and 13C Resonance Assignment of the PH Domain from C. elegans UNC-89"  J. Biomol. NMR 15, 269-270 (1999).

Assembly members:
PH domain from C. elegans UNC-89 protein, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PH domain from C. elegans UNC-89 protein: GRIIRHDAFQVWEGDEPPKL RYVFLFRNKIMFTEQDASTS PPSYTHYSSIRLDKYNIRQH TTDEDTIVLQPQEPGLPSFR IKPKDFETSEYVRKAWLRDI AEEQEKYAAERD

Data sets:
Data typeCount
13C chemical shifts231
15N chemical shifts99
1H chemical shifts669

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UNC-89 PH domain1

Entities:

Entity 1, UNC-89 PH domain 112 residues - Formula weight is not available

1   GLYARGILEILEARGHISASPALAPHEGLN
2   VALTRPGLUGLYASPGLUPROPROLYSLEU
3   ARGTYRVALPHELEUPHEARGASNLYSILE
4   METPHETHRGLUGLNASPALASERTHRSER
5   PROPROSERTYRTHRHISTYRSERSERILE
6   ARGLEUASPLYSTYRASNILEARGGLNHIS
7   THRTHRASPGLUASPTHRILEVALLEUGLN
8   PROGLNGLUPROGLYLEUPROSERPHEARG
9   ILELYSPROLYSASPPHEGLUTHRSERGLU
10   TYRVALARGLYSALATRPLEUARGASPILE
11   ALAGLUGLUGLNGLULYSTYRALAALAGLU
12   ARGASP

Samples:

sample_1: PH domain from C. elegans UNC-89 protein, [U-13C; U-15N], 0.6 – 0.8 mM; Pi 5 mM; NaCl 50 mM; d6-DMSO 16%

experimental_conditions_1: ionic strength: 0.13 M; pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQC NOESYsample_1not availableexperimental_conditions_1
1H-15N HSQC TOCSYsample_1not availableexperimental_conditions_1
CBCA(CO)NHsample_1not availableexperimental_conditions_1
CBCANHsample_1not availableexperimental_conditions_1
HNCAsample_1not availableexperimental_conditions_1
HN(CO)CAsample_1not availableexperimental_conditions_1
HBHA(CO)NHsample_1not availableexperimental_conditions_1
HCCH-TOCSYsample_1not availableexperimental_conditions_1
1H-13C HMQC NOESYsample_1not availableexperimental_conditions_1
2D 1H-1H NOESYsample_1not availableexperimental_conditions_1
HNHA-Jsample_1not availableexperimental_conditions_1

Software:

XWIN-NMR - Acquisition

Xeasy - Spectral analysis

NMRPipe - Processing

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
EMBL CCD63864 CCD63865 CCD63868 CCD63869 CCD63870
GB AAB00542
REF NP_001020984 NP_001020985 NP_001020988 NP_001020989 NP_001020990
SP O01761