BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4378

Title: 1H, 13C and 15N Resonance Assignment of un-myristoylated Ca2+-Frequenin, a Synaptic Efficacy Modulator

Authors: Kragelund, Birthe; Hauenschild, Alexander; Carlstrom, Goran; Pongs, Olaf; Finn, Bryan

Citation: Kragelund, Birthe; Hauenschild, Alexander; Carlstrom, Goran; Pongs, Olaf; Finn, Bryan. "Letter to the Editor: 1H, 13C, and 15N assignments of un-myristoylated Ca2+-frequenin, a synaptic efficacy modulator"  J. Biomol. NMR 16, 85-86 (2000).

Assembly members:
calcium loaded un-myristoylated rat frequenin, polymer, 190 residues, 21747.56 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calcium loaded un-myristoylated rat frequenin: MGKSNSKLKPEVVEELTRKT YFTEKEVQQWYKGFIKDCPS GQLDAAGFQKIYKQFFPFGD PTKFATFVFNVFDENKDGRI EFSEFIQALSVTSRGTLDEK LRWAFKLYDLDNDGYITRNE MLDIVDAIYQMVGNTVELPE EENTPEKRVDRIFAMMDKNA DGKLTLQEFQEGSKADPSIV QALSLYDGLV

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts721
15N chemical shifts182
1H chemical shifts1075

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rfrq1
2Ca-12
3Ca-22
4Ca-32

Entities:

Entity 1, rfrq 190 residues - 21747.56 Da.

1   METGLYLYSSERASNSERLYSLEULYSPRO
2   GLUVALVALGLUGLULEUTHRARGLYSTHR
3   TYRPHETHRGLULYSGLUVALGLNGLNTRP
4   TYRLYSGLYPHEILELYSASPCYSPROSER
5   GLYGLNLEUASPALAALAGLYPHEGLNLYS
6   ILETYRLYSGLNPHEPHEPROPHEGLYASP
7   PROTHRLYSPHEALATHRPHEVALPHEASN
8   VALPHEASPGLUASNLYSASPGLYARGILE
9   GLUPHESERGLUPHEILEGLNALALEUSER
10   VALTHRSERARGGLYTHRLEUASPGLULYS
11   LEUARGTRPALAPHELYSLEUTYRASPLEU
12   ASPASNASPGLYTYRILETHRARGASNGLU
13   METLEUASPILEVALASPALAILETYRGLN
14   METVALGLYASNTHRVALGLULEUPROGLU
15   GLUGLUASNTHRPROGLULYSARGVALASP
16   ARGILEPHEALAMETMETASPLYSASNALA
17   ASPGLYLYSLEUTHRLEUGLNGLUPHEGLN
18   GLUGLYSERLYSALAASPPROSERILEVAL
19   GLNALALEUSERLEUTYRASPGLYLEUVAL

Entity 2, Ca-1 - Ca - 40.078 Da.

1   CA

Samples:

sample_one: calcium loaded un-myristoylated rat frequenin, [U-99% 15N], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_two: calcium loaded un-myristoylated rat frequenin, [U-99% 13C], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_three: calcium loaded un-myristoylated rat frequenin, [U-99% 13C; U-99% 15N], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 10%; H2O 90%

sample_four: calcium loaded un-myristoylated rat frequenin, [U-99% 13C], 1.4 mM; CaCl2 8 mM; DTT 14 mM; NaN3 0.2%; D2O 100%

sample_conditions_one: pH: 7.2 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY-HSQCnot availablenot availablesample_conditions_one
3D 1H-15N TOCSY-HSQCnot availablenot availablesample_conditions_one
3D HNCAnot availablenot availablesample_conditions_one
3D HNCOnot availablenot availablesample_conditions_one
3D HNCOCAnot availablenot availablesample_conditions_one
3D CBCACOHNnot availablenot availablesample_conditions_one
3D CBCACOHAnot availablenot availablesample_conditions_one
3D HCCH-TOCSYnot availablenot availablesample_conditions_one
3D 1H-13C NOESY-HSQCnot availablenot availablesample_conditions_one
4D 1H-13C NOESY-HSQCnot availablenot availablesample_conditions_one
2D 1H-15N HSQCnot availablenot availablesample_conditions_one

Software:

PRONTO v19981230, Carlsberg A/S - assignment

NMR spectrometers:

  • Varian UNITYplus 600 MHz

Related Database Links:

BMRB 16334 16726
PDB
DBJ BAC37557 BAG73169
EMBL CAA57678 CAA58867 CAG05167 CAH90617 CAJ81731
GB AAA85633 AAA88510 AAC59690 AAD01642 AAF01804
REF NP_001018350 NP_001035637 NP_001084088 NP_001085136 NP_001122298
SP P62166 P62167 P62168 Q2V8Y7 Q5RC90
TPG DAA24173