BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4395

Title: RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI, NMR,

Authors: Stoldt, M.; Wohnert, J.; Gorlach, M.; Brown, L.

Citation: Stoldt, M.; Wohnert, J.; Gorlach, M.; Brown, L.. "The NMR Structure of Escherichia coli Ribosomal Protein L25 shows Homology to General Stress Proteins and Glutaminyl-tRNA Synthetases"  EMBO J. 17, 6377-6384 (1998).

Assembly members:
RIBOSOMAL PROTEIN L25, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: natural source   Host organism: Escherichia coli

Entity Sequences (FASTA):
RIBOSOMAL PROTEIN L25: MFTINAEVRKEQGKGASRRL RAANKFPAIIYGGKEAPLAI ELDHDKVMNMQAKAEFYSEV LTIVVDGKEIKVKAQDVQRH PYKPKLQHIDFVRA

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts93
1H chemical shifts675

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L25 monomer1

Entities:

Entity 1, L25 monomer 94 residues - Formula weight is not available

1   METPHETHRILEASNALAGLUVALARGLYS
2   GLUGLNGLYLYSGLYALASERARGARGLEU
3   ARGALAALAASNLYSPHEPROALAILEILE
4   TYRGLYGLYLYSGLUALAPROLEUALAILE
5   GLULEUASPHISASPLYSVALMETASNMET
6   GLNALALYSALAGLUPHETYRSERGLUVAL
7   LEUTHRILEVALVALASPGLYLYSGLUILE
8   LYSVALLYSALAGLNASPVALGLNARGHIS
9   PROTYRLYSPROLYSLEUGLNHISILEASP
10   PHEVALARGALA

Samples:

sample_1: RIBOSOMAL PROTEIN L25, [U-13C; U-15N], 1.4 mM; KCl 100 mM

sample_2: RIBOSOMAL PROTEIN L25, [U-15N], 1.4 mM; KCl 100 mM

sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_cond_1

Software:

DYANA v1.5 - structure calculation

NMR spectrometers:

  • Varian UNITY-INOVA 750 MHz

Related Database Links:

PDB
DBJ BAA02585 BAB36500 BAE76650 BAG77978 BAI26310
EMBL CAQ32591 CAQ99112 CAU98309 CBJ01826 CCJ44681
GB AAA16413 AAC75246 AAG57323 AAN43791 AAP17608
PRF 1712317A 754714A
REF NP_311104 NP_416690 NP_708084 WP_000494178 WP_000494179
SP A7ZP09 A8A248 B1IY84 B1X883 B2TV63