BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4401

Title: Structure and Interaction Site of the Regulatory Domain of Troponin-C when Complexed with the 96-148 Region of Troponin-I

Authors: McKay, R.; Pearlstone, J.; Corson, D.; Gagne, S.; Smillie, L.; Sykes, B.

Citation: McKay, R.; Pearlstone, J.; Corson, D.; Gagne, S.; Smillie, L.; Sykes, B.. "Structure and Interaction Site of the Regulatory Domain of Troponin-C when Complexed with the 96-148 Region of Troponin-I"  Biochemistry 37, 12419-12430 (1998).

Assembly members:
skeletal N-troponin C, polymer, 90 residues, 18000 Da.
sTnI115-131, polymer, 53 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
skeletal N-troponin C: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKEELDA IIEEVDEDGSGTIDFEEFLV MMVRQMKEDA

Data sets:
Data typeCount
13C chemical shifts324
15N chemical shifts88
1H chemical shifts542

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1skeletal N-Troponin C1
2TnI peptide2

Entities:

Entity 1, skeletal N-Troponin C 90 residues - 18000 Da.

1   ALASERMETTHRASPGLNGLNALAGLUALA
2   ARGALAPHELEUSERGLUGLUMETILEALA
3   GLUPHELYSALAALAPHEASPMETPHEASP
4   ALAASPGLYGLYGLYASPILESERTHRLYS
5   GLULEUGLYTHRVALMETARGMETLEUGLY
6   GLNASNPROTHRLYSGLUGLULEUASPALA
7   ILEILEGLUGLUVALASPGLUASPGLYSER
8   GLYTHRILEASPPHEGLUGLUPHELEUVAL
9   METMETVALARGGLNMETLYSGLUASPALA

Entity 2, TnI peptide 53 residues - Formula weight is not available

1   ASNGLNLYSLEUPHEASPLEUARGGLYLYS
2   PHELYSARGPROPROLEUARGARGVALARG
3   METSERALAASPALAMETLEULYSALALEU
4   LEUGLYSERLYSHISLYSVALALAMETASP
5   LEUARGALAASNLEULYSGLNVALLYSLYS
6   GLUASPTHR

Samples:

sample_1: skeletal N-troponin C, [U-98% 13C; U-98% 15N], 0.7 mM; KCl 100 mM

sample_2: skeletal N-troponin C, [U-98% 15N; U-88% 2H], 0.7 mM; KCl 100 mM

sample_3: skeletal N-troponin C, unlabeled, 0.9 mM; KCl 100 mM

sample_cond_1: ionic strength: 100 mM; pH: 6.85; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N-HMQCnot availablenot availablesample_cond_1
15N-HSQCnot availablenot availablesample_cond_1
TOCSY-hmqcnot availablenot availablesample_cond_1
HCCH-TOCSYnot availablenot availablesample_cond_1
CBCA(CO)NNHnot availablenot availablesample_cond_1
15N-noesyhsqcnot availablenot availablesample_cond_1
15N-noesyhmqcnot availablenot availablesample_cond_1
13C15N-noesynot availablenot availablesample_cond_1
HNCOnot availablenot availablesample_cond_1
HNCAnot availablenot availablesample_cond_1
HCACOnot availablenot availablesample_cond_1

Software:

NMRPipe - processing of data

PIPP - primitive interactive peak picker

NMR spectrometers:

  • Varian Unity-Inova 500 MHz
  • Varian Unity 600 MHz

Related Database Links:

BMRB 16414 4232
PDB
GB EMP28292 KFU86923
REF XP_007067817 XP_009321443 XP_009582608 XP_009693659 XP_009992324