BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4407

Title: NMR chemical shift assignment of human GAIP (Galpha Interacting Protein): A regulator of G protein signaling

Authors: de Alba, E.; De Vries, L.; Farquhar, M.; Tjandra, N.

Citation: de Alba, E.; De Vries, L.; Farquhar, M.; Tjandra, N.. "Solution structure of human GAIP (G alpha interacting protein). A regulator of G protein signaling"  J. Mol. Biol. 291, 927-939 (1999).

Assembly members:
Gaip protein, polymer, 153 residues, 17321 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Gaip protein: GSPGISGGGGGIPSPEEVQS WAQSFDKLMHSPAGRSVFRA FLRTEYSEENMLFWLACEEL KAEANQHVVDEKARLIYEDY VSILSPKEVSLDSRVREGIN KKMQEPSAHTFDDAQLQIYT LMHRDSYPRFLSSPTYRALL PWVDSSSSLIHRD

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts125
1H chemical shifts899

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gaip1

Entities:

Entity 1, gaip 153 residues - 17321 Da.

1   GLYSERPROGLYILESERGLYGLYGLYGLY
2   GLYILEPROSERPROGLUGLUVALGLNSER
3   TRPALAGLNSERPHEASPLYSLEUMETHIS
4   SERPROALAGLYARGSERVALPHEARGALA
5   PHELEUARGTHRGLUTYRSERGLUGLUASN
6   METLEUPHETRPLEUALACYSGLUGLULEU
7   LYSALAGLUALAASNGLNHISVALVALASP
8   GLULYSALAARGLEUILETYRGLUASPTYR
9   VALSERILELEUSERPROLYSGLUVALSER
10   LEUASPSERARGVALARGGLUGLYILEASN
11   LYSLYSMETGLNGLUPROSERALAHISTHR
12   PHEASPASPALAGLNLEUGLNILETYRTHR
13   LEUMETHISARGASPSERTYRPROARGPHE
14   LEUSERSERPROTHRTYRARGALALEULEU
15   PROTRPVALASPSERSERSERSERLEUILE
16   HISARGASP

Samples:

sample_1: Gaip protein, [U-13C; U-15N], 0.6 – 1.0 mM

sample_cond_1: ionic strength: 5 mM; pH: 7.2; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
CBCACONHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
C-CNOESYnot availablenot availablenot available
N-CNOESYnot availablenot availablenot available
H-HNOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
EMBL CAG33711
GB AAI23822 EDL07441 EDL07445 EPY87810
REF NP_001070383 XP_003462829 XP_003787844 XP_005214874 XP_006045501
SP Q08DC7
TPG DAA23284