BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4413

Title: STRUCTURE OF THE C-TERMINAL DOMAIN OF P73

Authors: Chi, Seung-Wook; Ayed, Ayeda; Arrowsmith, Cheryl

Citation: Chi, Seung-Wook; Ayed, Ayeda; Arrowsmith, Cheryl. "Solution structure of a conserved C-terminal domain of p73 with structural homology to the SAM domain"  EMBO J. 18, 4438-4445 (1999).

Assembly members:
p73, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
p73: MGSSHHHHHHSSGLVPRGSH MYHADPSLVSFLTGLGCPNC IEYFTSQGLQSIYHLQNLTI EDLGALKIPEQYRMTIWRGL QDLKQGHDY

Data sets:
Data typeCount
13C chemical shifts205
15N chemical shifts74
1H chemical shifts469

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p731

Entities:

Entity 1, p73 89 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METTYRHISALAASPPROSERLEUVALSER
4   PHELEUTHRGLYLEUGLYCYSPROASNCYS
5   ILEGLUTYRPHETHRSERGLNGLYLEUGLN
6   SERILETYRHISLEUGLNASNLEUTHRILE
7   GLUASPLEUGLYALALEULYSILEPROGLU
8   GLNTYRARGMETTHRILETRPARGGLYLEU
9   GLNASPLEULYSGLNGLYHISASPTYR

Samples:

sample_1: p73, [U-13C; U-15N], 1.2 mM; NaCl 150 mM; Sodium Phosphate 25 mM

sample_2: p73, [U-15N], 1.2 mM; NaCl 150 mM; Sodium Phosphate 25 mM

sample_cond_1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D C13,N15 edited NOESYnot availablenot availablesample_cond_1
2D 1H-15N HSQCnot availablenot availablesample_cond_1
3D 1H-1H-15N NOESYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
2D HSQCnot availablenot availablesample_cond_1
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablesample_cond_1
3D HNHAnot availablenot availablesample_cond_1
3D CBCANHnot availablenot availablesample_cond_1
3D HBCBCA(CO)NNHnot availablenot availablesample_cond_1
3D CCC-TOCSYnot availablenot availablesample_cond_1

Software:

NMRPipe - raw spectral data processing

NMRView - peak picking, analysis of processed data

InsightII - graphics generation

X-PLOR v3.851 - structure calculation

ARIA - structure calculation

ProcheckNMR - structure validation

GRASP - molecular surface generation

NMR spectrometers:

  • Varian Unity 600 MHz
  • Varian Unity+ 500 MHz

Related Database Links:

PDB
GB ELK10598 ELV14255
REF NP_001191117 NP_001191120 XP_002750246 XP_004024582 XP_004024584