BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4422

Title: NMR Solution Structure of Apis mellifera Chymotrypsin Inhibitor (AMCI).

Authors: Cierpicki, T.; Otlewski, J.

Citation: Cierpicki, Tomasz; Bania, Jacek; Otlewski, Jacek. "NMR Solution Structure of Apis mellifera Chymotrypsin/Cathepsin G Inhibitor-1 (AMCI-1): Structural Similarity with Ascaris Protease Inhibitors."  Protein Sci. 9, 976-984 (2000).

Assembly members:
Apis mellifera chymotrypsin inhibitor, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: Honeybee   Taxonomy ID: 7460   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Apis mellifera

Experimental source:   Production method: purified from natural source

Entity Sequences (FASTA):
Apis mellifera chymotrypsin inhibitor: EECGPNEVFNTCGSACAPTC AQPKTRICTMQCRIGCQCQE GFLRNGEGACVLPENC

Data sets:
Data typeCount
1H chemical shifts350

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AMCI1

Entities:

Entity 1, AMCI 56 residues - Formula weight is not available

1   GLUGLUCYSGLYPROASNGLUVALPHEASN
2   THRCYSGLYSERALACYSALAPROTHRCYS
3   ALAGLNPROLYSTHRARGILECYSTHRMET
4   GLNCYSARGILEGLYCYSGLNCYSGLNGLU
5   GLYPHELEUARGASNGLYGLUGLYALACYS
6   VALLEUPROGLUASNCYS

Samples:

sample_one: Apis mellifera chymotrypsin inhibitor 6.6 mM

sample_conditions: pH*: 2.5 na; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions
TOCSYsample_onenot availablesample_conditions
DQFCOSYsample_onenot availablesample_conditions

Software:

NMRPipe - spectra processing

SPARKY - analysis and intergation of NMR spectra

DYANA - structure calculation

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
REF XP_006563422
SP P56682