BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4426

Title: Solution Structure of Holo-biotinyl Domain from Acetyl Coenzyme A Carboxylase of Escherichia coli Determined by Triple-Resonance NMR Spectroscopy

Authors: Roberts, E.; Shu, N.; Howard, M.; Broadhurst, R.; Chapman-smith, A.; Wallace, J.; Morris, T.; Cronan, J.; Perham, R.

Citation: Roberts, E.; Shu, N.; Howard, M.; Broadhurst, R.; Chapman-smith, A.; Wallace, J.; Morris, T.; Cronan, J.; Perham, R.. "Solution Structure of Apo- and Holo-biotinyl Domain from Acetyl Coenzyme A Carboxylase of Escherichia coli Determined by Triple-Resonance NMR Spectroscopy"  Biochemistry 38, 5045-5053 (1999).

Assembly members:
ACETYL-COA CARBOXYLASE, polymer, 82 residues, Formula weight is not available
BIOTIN, non-polymer, 244.311 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ACETYL-COA CARBOXYLASE: AAEISGHIVRSPMVGTFYRT PSPDAKAFIEVGQKVNVGDT LCIVEAMKMMNQIEADKSGT VKAILVESGQPVEFDEPLVV IE

Data sets:
Data typeCount
1H chemical shifts531
13C chemical shifts269
15N chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acc biotinyl domain1
2btn2

Entities:

Entity 1, acc biotinyl domain 82 residues - Formula weight is not available

1   ALAALAGLUILESERGLYHISILEVALARG
2   SERPROMETVALGLYTHRPHETYRARGTHR
3   PROSERPROASPALALYSALAPHEILEGLU
4   VALGLYGLNLYSVALASNVALGLYASPTHR
5   LEUCYSILEVALGLUALAMETLYSMETMET
6   ASNGLNILEGLUALAASPLYSSERGLYTHR
7   VALLYSALAILELEUVALGLUSERGLYGLN
8   PROVALGLUPHEASPGLUPROLEUVALVAL
9   ILEGLU

Entity 2, btn - C10 H16 N2 O3 S - 244.311 Da.

1   BTN

Samples:

sample_one: ACETYL-COA CARBOXYLASE 3.0 mM

sample_conditions: pH: 6.8 na; temperature: 303 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_onenot availablesample_conditions
NOESYsample_onenot availablesample_conditions
15N-1H-HSQCsample_onenot availablesample_conditions
HNHAsample_onenot availablesample_conditions
HNHBsample_onenot availablesample_conditions
15N-NOESY-HMQCsample_onenot availablesample_conditions
15N-TOCSY-HMQCsample_onenot availablesample_conditions
HNCACBsample_onenot availablesample_conditions
13C-NOESY-HSQCsample_onenot availablesample_conditions
HCCH-TOCSYsample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 4425
PDB
DBJ BAB37550 BAE77296 BAG79060 BAI27525 BAI32704
EMBL CAA32933 CAD07894 CAP77706 CAQ33581 CAQ90726
GB AAA23408 AAA23744 AAA23745 AAA58058 AAC76287
PIR AH0912
REF NP_312154 NP_417721 NP_457755 NP_462289 NP_709050
SP P0ABD8 P0ABD9 P0ABE0 P0ABE1