BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4428

Title: Solution Structure of the Headpiece Domain of Chicken Villin   PubMed: 10600386

Authors: Vardar, D.; Buckley, D.; Frank, B.; McKnight, C.

Citation: Vardar, D.; Buckley, D.; Frank, B.; McKnight, C.. "NMR structure of an F-actin-binding "headpiece" motif from villin"  J. Mol. Biol. 294, 1299-1310 (1999).

Assembly members:
HEADPIECE DOMAIN FROM CHICKEN VILLIN, polymer, 67 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: .

Entity Sequences (FASTA):
HEADPIECE DOMAIN FROM CHICKEN VILLIN: PTKLETFPLDVLVNTAAEDL PRGVDPSRKENHLSDEDFKA VFGMTRSAFANLPLWKQQNL KKEKGLF

Data sets:
Data typeCount
1H chemical shifts456
15N chemical shifts69
coupling constants42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HEADPIECE DOMAIN FROM CHICKEN VILLIN1

Entities:

Entity 1, HEADPIECE DOMAIN FROM CHICKEN VILLIN 67 residues - Formula weight is not available

1   PROTHRLYSLEUGLUTHRPHEPROLEUASP
2   VALLEUVALASNTHRALAALAGLUASPLEU
3   PROARGGLYVALASPPROSERARGLYSGLU
4   ASNHISLEUSERASPGLUASPPHELYSALA
5   VALPHEGLYMETTHRARGSERALAPHEALA
6   ASNLEUPROLEUTRPLYSGLNGLNASNLEU
7   LYSLYSGLULYSGLYLEUPHE

Samples:

sample_1: HEADPIECE DOMAIN FROM CHICKEN VILLIN 1 mM; phosphate buffer 10 mM

sample_2: HEADPIECE DOMAIN FROM CHICKEN VILLIN, [U-15N], 1 mM; phosphate buffer 10 mM

sample_3: HEADPIECE DOMAIN FROM CHICKEN VILLIN, [U-15N; U-10% 13C], 1 mM; phosphate buffer 10 mM

sample_4: HEADPIECE DOMAIN FROM CHICKEN VILLIN 1 mM

sample_cond_1: pH: 7.0; temperature: 293 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
3D 15N-SEPARATED NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
E-COSYnot availablenot availablesample_cond_1
HNHBnot availablenot availablesample_cond_1

Software:

NMRPIPE -

X-PLOR -

XWINNMR -

NMRVIEW -

FELIX -

MOLMOL -

NMR spectrometers:

  • BRUKER DMX 500 MHz

Related Database Links:

BMRB 11519 11520 11521 15097 15245 17698
PDB
GB AAA49133
REF NP_990773 XP_003207697 XP_010711916 XP_010711917
SP P02640