BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4429

Title: 1H and 15N Chemical Shift Assignments for ribosomal protein L7

Authors: Bocharov, Eduard

Citation: Bocharov, Eduard; Gudkov, Anatolij; Arseniev, Alexandr. "Topology of the secondary structure elements of ribosomal protein L7/L12 from E.coli in solutio"  FEBS Lett. 379, 291-294 (1996).

Assembly members:
E.coli ribosomal protein L7/L12, polymer, 120 residues, 12164 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
E.coli ribosomal protein L7/L12: SITKDQIIEAVAAMSVMDVV ELISAMEEKFGVSAAAAVAV AAGPVEAAEEKTEFDVILKA AGANKVAVIKAVRGATGLGL KEAKDLVESAPAALKEGVSK DDAEALKKALEEAGAEVEVK

Data sets:
Data typeCount
1H chemical shifts823
15N chemical shifts120
coupling constants118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L7 dimer subunit A1
2L7 dimer subunit B1

Entities:

Entity 1, L7 dimer subunit A 120 residues - 12164 Da.

1   SERILETHRLYSASPGLNILEILEGLUALA
2   VALALAALAMETSERVALMETASPVALVAL
3   GLULEUILESERALAMETGLUGLULYSPHE
4   GLYVALSERALAALAALAALAVALALAVAL
5   ALAALAGLYPROVALGLUALAALAGLUGLU
6   LYSTHRGLUPHEASPVALILELEULYSALA
7   ALAGLYALAASNLYSVALALAVALILELYS
8   ALAVALARGGLYALATHRGLYLEUGLYLEU
9   LYSGLUALALYSASPLEUVALGLUSERALA
10   PROALAALALEULYSGLUGLYVALSERLYS
11   ASPASPALAGLUALALEULYSLYSALALEU
12   GLUGLUALAGLYALAGLUVALGLUVALLYS

Samples:

sample_1: E.coli ribosomal protein L7/L12, [U-95% 15N], 1.0 mM

sample_cond_1: pH: 6.9; temperature: 303 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HMQCsample_1not availablesample_cond_1
1H-15N HSQCsample_1not availablesample_cond_1
1H-15N TOCSY-HSQCsample_1not availablesample_cond_1
1H-15N NOESY-HSQCsample_1not availablesample_cond_1
1H TOCSYsample_1not availablesample_cond_1
1H NOESYsample_1not availablesample_cond_1

Software:

XEASY v1.2.11 - peak assignments

DYANA v1.5 - structure calculation

FANTOM v4 - energy minimization

MOLMOL v2.5.1 - visualization and structure analysis

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

PDB
DBJ BAB38332 BAE77334 BAG79797 BAH61123 BAI28246
EMBL CAA23624 CAA37246 CAD09488 CAP78442 CAQ34332
GB AAC43084 AAC76960 AAF33498 AAG59182 AAL22980
PIR AF0933
PRF 0601198A
REF NP_312936 NP_418413 NP_457918 NP_463021 NP_709781
SP A6TGN9 A7ZUK0 A8A785 A8AKU0 A9N0J3