BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4447

Title: Backbone assignment of the 19kDa translationally controlled tumor-associated protein p23fyp from Schizosaccharomyces pombe

Authors: Baxter, Nicola; Thaw, Paul; Higgins, Lee; Sedelnikova, Svetlana; Bramley, Alison; Price, Clive; Waltho, Jonathan; Craven, C Jeremy

Citation: Baxter, Nicola; Thaw, Paul; Higgins, Lee; Sedelnikova, Svetlana; Bramley, Alison; Price, Clive; Waltho, Jonathan; Craven, C Jeremy. "Letter to the Editor: Backbone assignment of the 19kDa translationally controlled tumor-associated protein p23 fyp from Schizosaccharomyces pombe"  J. Biomol. NMR 16, 83-84 (2000).

Assembly members:
p23fyp, polymer, 168 residues, Formula weight is not available

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
p23fyp: MLLYKDVISGDELVSDAYDL KEVDDIVYEADCQMVTVKQG GDVDIGANPSAEDAEENAEE GTETVNNLVYSFRLSPTSFD KKSYMSYIKGYMKAIKARLQ ESNPERVPVFEKNAIGFVKK ILANFKDYDFYIGESMDPDA MVVLMNYREDGITPYMIFFK DGLVSEKF

Data sets:
Data typeCount
1H chemical shifts338
13C chemical shifts494
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p23fyp1

Entities:

Entity 1, p23fyp 168 residues - Formula weight is not available

1   METLEULEUTYRLYSASPVALILESERGLY
2   ASPGLULEUVALSERASPALATYRASPLEU
3   LYSGLUVALASPASPILEVALTYRGLUALA
4   ASPCYSGLNMETVALTHRVALLYSGLNGLY
5   GLYASPVALASPILEGLYALAASNPROSER
6   ALAGLUASPALAGLUGLUASNALAGLUGLU
7   GLYTHRGLUTHRVALASNASNLEUVALTYR
8   SERPHEARGLEUSERPROTHRSERPHEASP
9   LYSLYSSERTYRMETSERTYRILELYSGLY
10   TYRMETLYSALAILELYSALAARGLEUGLN
11   GLUSERASNPROGLUARGVALPROVALPHE
12   GLULYSASNALAILEGLYPHEVALLYSLYS
13   ILELEUALAASNPHELYSASPTYRASPPHE
14   TYRILEGLYGLUSERMETASPPROASPALA
15   METVALVALLEUMETASNTYRARGGLUASP
16   GLYILETHRPROTYRMETILEPHEPHELYS
17   ASPGLYLEUVALSERGLULYSPHE

Samples:

sample_1: p23fyp, [U-13C; U-15N], 1 mM; sodium acetate, [U-2H], 20 mM; sodium chloride 250 mM; sodium azide 5 mM; PMSF 1 mM

Ex-cond_1: pH: 5.1; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CAA93806
REF NP_594328
SP Q10344