BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4452

Title: The Solution structure of Type II Antifreeze Protein Reveals a New Member of the Lectin Family

Authors: Gronwald, W.; Loewen, M.; Lix, B.; Daugulis, A.; Sonnichsen, F.; Davies, P.; Sykes, B.

Citation: Gronwald, W.; Loewen, M.; Lix, B.; Daugulis, A.; Sonnichsen, F.; Davies, P.; Sykes, B.. "The Solution Structure of Type II Antifreeze Protein Reveals a New Member of the Lectin Family"  Biochemistry 37, 4712-4721 (1998).

Assembly members:
sea raven Type II antifreeze protein, polymer, 129 residues, 13993 Da.

Natural source:   Common Name: Sea raven   Taxonomy ID: 8094   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hemitripterus americanus

Experimental source:   Production method: recombinant technology   Host organism: Pichia Pastoris

Entity Sequences (FASTA):
sea raven Type II antifreeze protein: QRAGPNCPAGWQPLGDRCIY YETTAMTWALAETNCMKLGG HLASIHSQEEHSFIQTLNAG VVWIGGSACLQAGAWTWSDG TPMNFRSWCSTKPDDVLAAC CMQMTAAADQCWDDLPCPAS HKSVCAMTF

Data sets:
Data typeCount
15N chemical shifts120
1H chemical shifts662

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SRAFP1

Entities:

Entity 1, SRAFP 129 residues - 13993 Da.

1   GLNARGALAGLYPROASNCYSPROALAGLY
2   TRPGLNPROLEUGLYASPARGCYSILETYR
3   TYRGLUTHRTHRALAMETTHRTRPALALEU
4   ALAGLUTHRASNCYSMETLYSLEUGLYGLY
5   HISLEUALASERILEHISSERGLNGLUGLU
6   HISSERPHEILEGLNTHRLEUASNALAGLY
7   VALVALTRPILEGLYGLYSERALACYSLEU
8   GLNALAGLYALATRPTHRTRPSERASPGLY
9   THRPROMETASNPHEARGSERTRPCYSSER
10   THRLYSPROASPASPVALLEUALAALACYS
11   CYSMETGLNMETTHRALAALAALAASPGLN
12   CYSTRPASPASPLEUPROCYSPROALASER
13   HISLYSSERVALCYSALAMETTHRPHE

Samples:

sample_1: sea raven Type II antifreeze protein, [U-15N], 0.5 – 1.2 mM

sample_cond_1: pH: 5.6; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D-DQF-COSYsample_1not availablesample_cond_1
2D-TOCSYsample_1not availablesample_cond_1
2D-NOESYsample_1not availablesample_cond_1
3D-TOCSY-HSQCsample_1not availablesample_cond_1
3D-NOESY-HSQCsample_1not availablesample_cond_1
3D-HNHAsample_1not availablesample_cond_1

Software:

X-PLOR v3.1 - structure calculation

VNMR v5.1 - Acquisition and processing of NMR spectra

NMRPipe - Processing of NMR spectra

PIPP - Analysis of NMR spectra

NMR spectrometers:

  • Varian Unity 600 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

PDB
GB AAA49617 AAA49618
SP P05140