BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4461

Title: 1H NMR Solution Structure of Cycloviolacin O1 - A Macro-cyclic Polypeptide Isolated from Viola odorat   PubMed: 10600388

Authors: Craik, David; Daly, Norelle; Bond, Trudy; Waine, Clement

Citation: Craik, David; Daly, Norelle; Bond, Trudy; Waine, Clement. "Plant Cyclotides - a unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural moti"  J. Mol. Biol. 294, 1327-1336 (1999).

Assembly members:
cyclotide, polymer, 30 residues, 3116 Da.

Natural source:   Common Name: Viola   Taxonomy ID: 97441   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Violaceae odorata

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
cyclotide: SCVYIPCTVTALLGCSCSNR VCYNGIPCAE

Data sets:
Data typeCount
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cck1

Entities:

Entity 1, cck 30 residues - 3116 Da.

1   SERCYSVALTYRILEPROCYSTHRVALTHR
2   ALALEULEUGLYCYSSERCYSSERASNARG
3   VALCYSTYRASNGLYILEPROCYSALAGLU

Samples:

ss1559: cyclotide 1.6 mM

ss1559-cond_1: pH: 3.6 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H E-COSYnot availablenot availablenot available
2D 1H-1H DQF-COSYnot availablenot availablenot available

Software:

ProcheckNMR - Ramachandran plot

X-PLOR - Structure Calculations and energy minimizations

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
SP P82230