BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4467

Title: Sequence-specific 1H, 13C, and 15N Assignments of the MAR-binding Domain of Chicken MeCP2/ARBP

Authors: Brunner, Eike; Weitzel, Joachim; Heitmann, Bjoern; Maurer, Till; Straetling, Wolf; Kalbitzer, Hans Robert

Citation: Brunner, Eike; Weitzel, Joachim; Heitmann, Bjoern; Maurer, Till; Straetling, Wolf; Kalbitzer, Hans Robert. "Sequence-specific 1H, 13C, and 15N Assignments of the MAR-binding Domain of Chicken MeCP2/ARBP"  J. Biomol. NMR 17, 175-176 (2000).

Assembly members:
Methyl-CpG-binding protein, attachment region binding protein, polymer, 125 residues, 16300 Da.

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Methyl-CpG-binding protein, attachment region binding protein: APAVPEASASPKQRRSIIRD RGPMYDDPTLPEGWTRKLKQ RKSGRSAGKYDVYLINPQGK AFRSKVELIAYFEKVGDTSL DPNDFDFTVTGRGSPSRREQ RPPKKAKSPKSPGSGRGRGR PKGSG

Data sets:
Data typeCount
1H chemical shifts231
13C chemical shifts319
15N chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MeCP2/ARBP1

Entities:

Entity 1, MeCP2/ARBP 125 residues - 16300 Da.

1   ALAPROALAVALPROGLUALASERALASER
2   PROLYSGLNARGARGSERILEILEARGASP
3   ARGGLYPROMETTYRASPASPPROTHRLEU
4   PROGLUGLYTRPTHRARGLYSLEULYSGLN
5   ARGLYSSERGLYARGSERALAGLYLYSTYR
6   ASPVALTYRLEUILEASNPROGLNGLYLYS
7   ALAPHEARGSERLYSVALGLULEUILEALA
8   TYRPHEGLULYSVALGLYASPTHRSERLEU
9   ASPPROASNASPPHEASPPHETHRVALTHR
10   GLYARGGLYSERPROSERARGARGGLUGLN
11   ARGPROPROLYSLYSALALYSSERPROLYS
12   SERPROGLYSERGLYARGGLYARGGLYARG
13   PROLYSGLYSERGLY

Samples:

sample_1: Methyl-CpG-binding protein, attachment region binding protein, [U-15N; U-13C], 2.0 mM

sample_2: Methyl-CpG-binding protein, attachment region binding protein, [U-15N], 2.0 mM

sample_3: Methyl-CpG-binding protein, attachment region binding protein 2.0 mM

sample_4: Methyl-CpG-binding protein, attachment region binding protein, [U-2H; U-13C; U-15N], 2.0 mM

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
1H-15N-HSQCnot availablenot availableEx-cond_1
1H-15N-NOESY HSQCnot availablenot availableEx-cond_1
1H-15N-TOCSY HSQCnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1

Software:

AURELIA v2.5 - visualisation

XWINNMR v2.5 - processing

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4280
PDB
EMBL CAA74577
GB EPY78157
REF XP_004645154 XP_004645155 XP_006113271 XP_007054114 XP_008175578