BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4473

Title: The molecular basis for Protein Kinase A anchoring revealed by solution NMR

Authors: Newlon, M.; Roy, M.; Morikis, D.; Hausken, Z.; Coghlan, V.; Scott, J.; Jennings, P.

Citation: NEWLON, M.; ROY, M.; MORIKIS, D.; HAUSKEN, Z.; COGHLAN, V.; SCOTT, J.; JENNINGS, P.. "The Molecular Basis for Protein Kinase A Anchoring Revealed by Solution NMR"  Nat. Struct. Biol. 6, 222-227 (1999).

Assembly members:
Camp dependent protein kinase type ii regulatory chain, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Camp dependent protein kinase type ii regulatory chain: HMGHIQIPPGLTELLQGYTV EVLRQQPPDLVDFAVEYFTR LREARR

Data sets:
Data typeCount
1H chemical shifts305
13C chemical shifts156
15N chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chain A1
2chain B1

Entities:

Entity 1, chain A 46 residues - Formula weight is not available

1   HISMETGLYHISILEGLNILEPROPROGLY
2   LEUTHRGLULEULEUGLNGLYTYRTHRVAL
3   GLUVALLEUARGGLNGLNPROPROASPLEU
4   VALASPPHEALAVALGLUTYRPHETHRARG
5   LEUARGGLUALAARGARG

Samples:

sample_1: Camp dependent protein kinase type ii regulatory chain

sample_cond_1: pH: 4.0; temperature: 298 K; ionic strength: 0.012 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D NOESY-HSQCnot availablenot availablenot available
3D TOCSY-HMQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCA,HN(CO)CAnot availablenot availablenot available
13C-filtered NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
GB AAM97689 ABK42343 EDL77146 EDL77147 EDL77148
REF NP_062137 XP_005075011 XP_005347993 XP_006243774 XP_006978360
SP P12368