BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4492

Title: Unmyristoylated GCAP-2 with three calcium ions bound   PubMed: 10383444

Authors: Ames, J.; Dizhoor, A.; Ikura, M.; Palczewski, K.; Stryer, L.

Citation: Ames, J.; Dizhoor, A.; Ikura, M.; Palczewski, K.; Stryer, L.. "Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases"  J. Biol. Chem. 274, 19329-19337 (1999).

Assembly members:
guanylate cyclase activating protein 2, polymer, 204 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
guanylate cyclase activating protein 2: MGQQFSWEEAEENGAVGAAD AAQLQEWYKKFLEECPSGTL FMHEFKRFFKVPDNEEATQY VEAMFRAFDTNGDNTIDFLE YVAALNLVLRGTLEHKLKWT FKIYDKDRNGCIDRQELLDI VESIYKLKKACSVEVEAEQQ GKLLTPEEVVDRIFLLVDEN GDGQLSLNEFVEGARRDKWV MKMLQMDLNPSSWISQQRRK SAMF

Data sets:
Data typeCount
1H chemical shifts675
13C chemical shifts558
15N chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCAP-21
2Ca2+ 12
3Ca2+ 22
4Ca2+ 32

Entities:

Entity 1, GCAP-2 204 residues - Formula weight is not available

1   METGLYGLNGLNPHESERTRPGLUGLUALA
2   GLUGLUASNGLYALAVALGLYALAALAASP
3   ALAALAGLNLEUGLNGLUTRPTYRLYSLYS
4   PHELEUGLUGLUCYSPROSERGLYTHRLEU
5   PHEMETHISGLUPHELYSARGPHEPHELYS
6   VALPROASPASNGLUGLUALATHRGLNTYR
7   VALGLUALAMETPHEARGALAPHEASPTHR
8   ASNGLYASPASNTHRILEASPPHELEUGLU
9   TYRVALALAALALEUASNLEUVALLEUARG
10   GLYTHRLEUGLUHISLYSLEULYSTRPTHR
11   PHELYSILETYRASPLYSASPARGASNGLY
12   CYSILEASPARGGLNGLULEULEUASPILE
13   VALGLUSERILETYRLYSLEULYSLYSALA
14   CYSSERVALGLUVALGLUALAGLUGLNGLN
15   GLYLYSLEULEUTHRPROGLUGLUVALVAL
16   ASPARGILEPHELEULEUVALASPGLUASN
17   GLYASPGLYGLNLEUSERLEUASNGLUPHE
18   VALGLUGLYALAARGARGASPLYSTRPVAL
19   METLYSMETLEUGLNMETASPLEUASNPRO
20   SERSERTRPILESERGLNGLNARGARGLYS
21   SERALAMETPHE

Entity 2, Ca2+ 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: guanylate cyclase activating protein 2, [U-15N], 1 mM; KCl 50 mM; CaCl2 10 mM; [2H10]dithiothreitol 10 mM; [2H28]octyl-glucoside 25 mM

sample_2: guanylate cyclase activating protein 2, [U-13C; U-15N], 1 mM; KCl 50 mM; CaCl2 10 mM; [2H10]dithiothreitol 10 mM; [2H28]octyl-glucoside 25 mM

sample_cond_1: pH: 6.8; temperature: 318 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablesample_cond_1
HNCACBnot availablenot availablesample_cond_1
CBCACONHnot availablenot availablesample_cond_1
NOESYnot availablenot availablesample_cond_1
TOCSYnot availablenot availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity-Plus 500 MHz

Related Database Links:

BMRB 25199
PDB
GB AAA83214 AAC48478 ELR53264
REF NP_777211 XP_005900200 XP_006069256 XP_010816471 XP_010861473
SP P51177
TPG DAA16519