BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4494

Title: Solution Structure and Backbone Dynamics of Human Long-[Arg3]Insulin-Like Growth Factor 1

Authors: Laajoki, L.; Francis, G.; Wallace, J.; Carver, J.; Keniry, M.

Citation: Laajoki, L.; Francis, G.; Wallace, J.; Carver, J.; Keniry, M.. "Solution Structure and Backbone Dynamics of Human Long-[Arg3]-Insulin Like Growth factor-1"  J. Biol. Chem. 275, 10009-10015 (2000).

Assembly members:
IGF-I, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IGF-I: MFPAMPLSSLFVNGPRTLCG AELVDALQFVCGDRGFYFNK PTGYGSSSRRAPQTGIVDEC CFRSCDLRRLEMYCAPLKPA KSA

Data sets:
Data typeCount
1H chemical shifts498
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IGF-I1

Entities:

Entity 1, IGF-I 83 residues - Formula weight is not available

1   METPHEPROALAMETPROLEUSERSERLEU
2   PHEVALASNGLYPROARGTHRLEUCYSGLY
3   ALAGLULEUVALASPALALEUGLNPHEVAL
4   CYSGLYASPARGGLYPHETYRPHEASNLYS
5   PROTHRGLYTYRGLYSERSERSERARGARG
6   ALAPROGLNTHRGLYILEVALASPGLUCYS
7   CYSPHEARGSERCYSASPLEUARGARGLEU
8   GLUMETTYRCYSALAPROLEULYSPROALA
9   LYSSERALA

Samples:

sample_1: IGF-I 1.0 mM

sample_cond_1: pH: 3.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-NOESY-HSQCnot availablenot availablenot available
3D 15N-TOCSY-HSQCnot availablenot availablenot available
3D 15N-HSQC-NOESY-HSQCnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D 15N-HSQC-TOCSYnot availablenot availablenot available
2D 15N-HSQC-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15654 2498 4069 4204 4278
PDB
DBJ BAA01200 BAA07897
EMBL CAA27153 CAA28759 CAA35098
GB AAA31544 AAA53535 AAA72202 AAA72204 AAA72455
PIR S22878
PRF 0912651A 1707201A 2001274B
REF NP_001300784 XP_004418088 XP_004418089 XP_004418090 XP_004418091
SP P33712 Q28933