BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4496

Title: Solution nmr structure of the mitochondrial protein import receptor Tom20 from rat in a complex with a presequence peptide derived from rat aldehyde dehydrogenase (ALDH)   PubMed: 10721992

Authors: Abe, Y.; Shodai, T.; Muto, T.; Mihara, K.; Torii, H.; Nishikawa, S.; Endo, T.; Kohda, D.

Citation: Abe, Y.; Shodai, T.; Muto, T.; Mihara, K.; Torii, H.; Nishikawa, S.; Endo, T.; Kohda, D.. "Structure Basis of Presequence Recognition by the Mitochondrial Protein Import Receptor Tom20"  Cell 100, 551-560 (2000).

Assembly members:
Tom20, polymer, 95 residues, Formula weight is not available

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Tom20: RAGLSKLPDLKDAEAVQKFF LEEIQLGEELLAQGDYEKGV DHLTNAIAVCGQPQQLLQVL QQTLPPPVFQMLLTKLPTIS QRIVSAQSLGEDDVE

Data sets:
Data typeCount
1H chemical shifts711
13C chemical shifts416
15N chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tom201

Entities:

Entity 1, Tom20 95 residues - Formula weight is not available

1   ARGALAGLYLEUSERLYSLEUPROASPLEU
2   LYSASPALAGLUALAVALGLNLYSPHEPHE
3   LEUGLUGLUILEGLNLEUGLYGLUGLULEU
4   LEUALAGLNGLYASPTYRGLULYSGLYVAL
5   ASPHISLEUTHRASNALAILEALAVALCYS
6   GLYGLNPROGLNGLNLEULEUGLNVALLEU
7   GLNGLNTHRLEUPROPROPROVALPHEGLN
8   METLEULEUTHRLYSLEUPROTHRILESER
9   GLNARGILEVALSERALAGLNSERLEUGLY
10   GLUASPASPVALGLU

Samples:

sample_1: Tom20, [U-13C; U-15N], 0.5 mM

sample_cond_1: pH: 6.8; temperature: 303 K; ionic strength: 0.02 M; pressure: 1 atm

sample_cond_2: pH: 5.4; temperature: 303 K; ionic strength: 0.02 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCA, HN(CO)CAsample_1not availablenot available
CBCA(CO)NHsample_1not availablenot available
HNCACBsample_1not availablenot available
HBHA(CO)NHsample_1not availablenot available
C(CO)NH-TOCSY HC(CO)NH-TOCSYsample_1not availablenot available
HCCH-COSY, HCCH-TOCSYsample_1not availablenot available
15N-resolved NOESYsample_1not availablenot available
15N-resolved TOCSYsample_1not availablenot available
NOESYsample_1not availablenot available
TOCSYsample_1not availablenot available
HNHAsample_1not availablenot available
{1H}-15N NOEsample_1not availablenot available
3D F1 13C-filtered-F3 13C-resolved NOESYsample_1not availablenot available
2D F1/F2 13C-filtered NOESYsample_1not availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAA02804 BAA09714 BAB22444 BAE24663 BAE35669
EMBL CAH91379
GB AAB01506 AAB35420 AAF13354 AAH00882 AAH02087
REF NP_001092653 NP_001127411 NP_001232519 NP_001247980 NP_055580
SP A6H7B1 Q15388 Q5RA31 Q62760 Q9DCC8
TPG DAA14365