BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4510

Title: The Second Type II Module From Human Matrix Metalloproteinase 2

Authors: Briknarova, Klara; Grishaev, Alexander; Banyai, Laszlo; Tordai, Hedvig; Patthy, Laszlo; Llinas, Miguel

Citation: Briknarova, Klara; Grishaev, Alexander; Banyai, Laszlo; Tordai, Hedvig; Patthy, Laszlo; Llinas, Miguel. "The second type II module from human matrix metalloproteinase 2: structure, function and dynamics"  Structure 7, 1235-1245 (1999).

Assembly members:
The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2: TALFTMGGNAEGQPCKFPFR FQGTSYDSCTTEGRTDGYRW CGTTEDYDRDKKYGFCPETA

Data sets:
Data typeCount
15N chemical shifts72
1H chemical shifts401

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12Fn2/MMP-21

Entities:

Entity 1, 2Fn2/MMP-2 60 residues - Formula weight is not available

1   THRALALEUPHETHRMETGLYGLYASNALA
2   GLUGLYGLNPROCYSLYSPHEPROPHEARG
3   PHEGLNGLYTHRSERTYRASPSERCYSTHR
4   THRGLUGLYARGTHRASPGLYTYRARGTRP
5   CYSGLYTHRTHRGLUASPTYRASPARGASP
6   LYSLYSTYRGLYPHECYSPROGLUTHRALA

Samples:

sample_1: The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2 1.3 mM; H2O 90%; D2O 10%

sample_2: The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, [U-15N], 1 mM; H2O 90%; D2O 10%

sample_3: The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, [U-15N], 4 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0 M; pH: 5.2; pressure: 1 atm; temperature: 298 K

sample_cond_2: ionic strength: 0 M; pH*: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
E-COSYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D 15N-edited TOCSYnot availablenot availablenot available
3D 15N-edited NOESYnot availablenot availablenot available
1N-15N NOEnot availablenot availablenot available
T1 and T2 relaxationnot availablenot availablenot available

Software:

xwinnmr v2.0 - experiment acquisition

FELIX v95 - data processing and assignment

X-PLOR v3.851 - structure calculation

QUANTA v96 - REFINEMENT

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
REF XP_011786088