BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4522

Title: The restrained and minimized average NMR structure of MAP30.

Authors: Wang, Y.; Neamati, N.; Jacob, J.; Palmer, I.; Stahl, S.

Citation: Wang, Y.; Neamati, N.; Jacob, J.; Palmer, I.; Stahl, S.. "Solution Structure of Anti-HIV-1 and Anti-Tumor Protein Map30: Structural Insights Into its Multiple Functions "  Cell 99, 433-442 (1999).

Assembly members:
MAP30, polymer, 263 residues, Formula weight is not available

Natural source:   Common Name: balsam pear   Taxonomy ID: 3673   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Momordica charantia

Experimental source:   Production method: .

Entity Sequences (FASTA):
MAP30: DVNFDLSTATAKTYTKFIED FRATLPFSHKVYDIPLLYST ISDSRRFILLNLTSYAYETI SVAIDVTNVYVVAYRTRDVS YFFKESPPEAYNILFKGTRK ITLPYTGNYENLQTAAHKIR ENIDLGLPALSSAITTLFYY NAQSAPSALLVLIQTTAEAA RFKYIERHVAKYVATNFKPN LAIISLENQWSALSKQIFLA QNQGGKFRNPVDLIKPTGER FQVTNVDSDVVKGNIKLLLN SRASTADENFITTMTLLGES VVN

Data sets:
Data typeCount
residual dipolar couplings387

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAP301

Entities:

Entity 1, MAP30 263 residues - Formula weight is not available

1   ASPVALASNPHEASPLEUSERTHRALATHR
2   ALALYSTHRTYRTHRLYSPHEILEGLUASP
3   PHEARGALATHRLEUPROPHESERHISLYS
4   VALTYRASPILEPROLEULEUTYRSERTHR
5   ILESERASPSERARGARGPHEILELEULEU
6   ASNLEUTHRSERTYRALATYRGLUTHRILE
7   SERVALALAILEASPVALTHRASNVALTYR
8   VALVALALATYRARGTHRARGASPVALSER
9   TYRPHEPHELYSGLUSERPROPROGLUALA
10   TYRASNILELEUPHELYSGLYTHRARGLYS
11   ILETHRLEUPROTYRTHRGLYASNTYRGLU
12   ASNLEUGLNTHRALAALAHISLYSILEARG
13   GLUASNILEASPLEUGLYLEUPROALALEU
14   SERSERALAILETHRTHRLEUPHETYRTYR
15   ASNALAGLNSERALAPROSERALALEULEU
16   VALLEUILEGLNTHRTHRALAGLUALAALA
17   ARGPHELYSTYRILEGLUARGHISVALALA
18   LYSTYRVALALATHRASNPHELYSPROASN
19   LEUALAILEILESERLEUGLUASNGLNTRP
20   SERALALEUSERLYSGLNILEPHELEUALA
21   GLNASNGLNGLYGLYLYSPHEARGASNPRO
22   VALASPLEUILELYSPROTHRGLYGLUARG
23   PHEGLNVALTHRASNVALASPSERASPVAL
24   VALLYSGLYASNILELYSLEULEULEUASN
25   SERARGALASERTHRALAASPGLUASNPHE
26   ILETHRTHRMETTHRLEULEUGLYGLUSER
27   VALVALASN

Samples:

sample_1: MAP30, [U-15N; U-13C], 0.7 mM

sample_cond_1: pH: 5.50; temperature: 313.00 K; ionic strength: 0.01 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-SEPARATED NOESYsample_1not availablesample_cond_1
4D 13C/15N-SEPARATED NOESYsample_1not availablesample_cond_1
3D 13C-SEPARATED NOESYsample_1not availablesample_cond_1
4D 13C-SEPARATED NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
HNCA-Jsample_1not availablesample_cond_1

Software:

MODIFIED XPLOR v3.5 - refinement, structure solution

NMR spectrometers:

  • BRUKER DMX 750 MHz

Related Database Links:

PDB
EMBL CAA78166 CAC08217 CAH19208 CCD28523 CCD28524
GB AAB35194 AAG33028 AAS17014 AAV68558 ABG37691
SP P24817 P29339