BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4526

Title: SOLUTION NMR STRUCTURE OF TUMOR SUPPRESSOR P16INK4A, 20 STRUCTURES   PubMed: 10892805

Authors: Yuan, C.; Selby, T.; Li, J.; Byeon, I.; Tsai, M.

Citation: Yuan, C.; Selby, T.; Li, J.; Byeon, I.; Tsai, M.. "Tumor suppressor INK4: refinement of p16INK4A structure and determination of p15INK4B structure by comparative modeling and NMR data"  Protein Sci. 9, 1120-1128 (2000).

Assembly members:
CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A): MEPAAGSSMEPSADWLATAA ARGRVEEVRALLEAGALPNA PNSYGRRPIQVMMMGSARVA ELLLLHGAEPNCADPATLTR PVHDAAREGFLDTLVVLHRA GARLDVRDAWGRLPVDLAEE LGHRDVARYLRAAAGGTRGS NHARIDAAEGPSDIPD

Data sets:
Data typeCount
13C chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P16INK4A1

Entities:

Entity 1, P16INK4A 156 residues - Formula weight is not available

1   METGLUPROALAALAGLYSERSERMETGLU
2   PROSERALAASPTRPLEUALATHRALAALA
3   ALAARGGLYARGVALGLUGLUVALARGALA
4   LEULEUGLUALAGLYALALEUPROASNALA
5   PROASNSERTYRGLYARGARGPROILEGLN
6   VALMETMETMETGLYSERALAARGVALALA
7   GLULEULEULEULEUHISGLYALAGLUPRO
8   ASNCYSALAASPPROALATHRLEUTHRARG
9   PROVALHISASPALAALAARGGLUGLYPHE
10   LEUASPTHRLEUVALVALLEUHISARGALA
11   GLYALAARGLEUASPVALARGASPALATRP
12   GLYARGLEUPROVALASPLEUALAGLUGLU
13   LEUGLYHISARGASPVALALAARGTYRLEU
14   ARGALAALAALAGLYGLYTHRARGGLYSER
15   ASNHISALAARGILEASPALAALAGLUGLY
16   PROSERASPILEPROASP

Samples:

sample_1: CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), [U-15N; U-13C], 0.2 – 0.4 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; H2O 95%; D2O 5%

sample_2: CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A), [U-15N], 0.2 – 0.4 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; H2O 95%; D2O 5%

sample_3: CYCLIN-DEPENDENT KINASE 4 INHIBITOR A (P16INK4A)0.2 – 0.4 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; H2O 95%; D2O 5%

sample_cond_1: pH: 7.5; temperature: 293 K; ionic strength: 0 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D_13C-SEPARATED NOESYnot availablenot availablenot available
4D_13C-SEPARATED NOESYnot availablenot availablenot available
4D_13C/15N-SEPARATED NOESYnot availablenot availablenot available
3D_15N-SEPARATED NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

X-PLOR v3.85 - STRUCTURE SOLUTION

XWINNMR v2.1 - COLLECTION, PROCESSING

FELIX v95 - PROCESSING

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4086
PDB
DBJ BAA33541 BAB91133
EMBL CCQ43815
GB AAA92554 AAB32713 AAB60645 AAD14048 AAD14050
PRF 2002364A
REF NP_000068 NP_001139762 NP_001182061 XP_003830733 XP_005251400
SP P42771