BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4536

Title: Structural basis for uracil DNA glycosylase interaction with uracil: NMR study

Authors: Ghosh, M.; Kumar1, V.; Varshney, U.; Chary, K.

Citation: Ghosh, M.; Kumar1, V.; Varshney, U.; Chary, K.. "Structural basis for uracil DNA glycosylase interaction with uracil: NMR study"  Nucleic Acids Res. 28, 1906-1912 (2000).

Assembly members:
URACIL CONTAINING HAIRPIN DNA, polymer, 22 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: not reported

Entity Sequences (FASTA):
URACIL CONTAINING HAIRPIN DNA: CTAGAGGATCCTXTTGGATC CT

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA hairpin1

Entities:

Entity 1, DNA hairpin 22 residues - Formula weight is not available

1   DCDTDADGDADGDGDADTDC
2   DCDTDURDTDTDGDGDADTDC
3   DCDT

Samples:

sample_1: URACIL CONTAINING HAIRPIN DNA 5 mM; D2O 99%

sample_2: URACIL CONTAINING HAIRPIN DNA 5 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.0; pressure: 1 atm; temperature: 305 K

sample_cond_2: pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

VNMR v6.1 - COLLECTION, DATA ANALYSIS

DISCOVER v97 - STRUCTURE SOLUTION

UXNMR v3.0 - COLLECTION

FELIX v97 - DATA ANALYSIS, PROCESSING

NMR spectrometers:

  • BRUKER AMX 500 MHz

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