BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4542

Title: Solution structure of a uracil containing hairpin DNA   PubMed: 10481034

Authors: Ghosh, M.; Kumar, N.; Varshney, U.; Chary, K.

Citation: Ghosh, M.; Kumar, N.; Varshney, U.; Chary, K.. "Structural Characterization of a Uracil Containing Hairpin DNA by NMR and Molecular Dynamics"  Nucleic Acids Res. 27, 3938-3944 (1999).

Assembly members:
5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3', polymer, 22 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: .

Entity Sequences (FASTA):
5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3': CTAGAGGATCCTTTXGGATC CT

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3'1

Entities:

Entity 1, 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3' 22 residues - Formula weight is not available

1   DCDTDADGDADGDGDADTDC
2   DCDTDTDTDUDGDGDADTDC
3   DCDT

Samples:

sample_1: 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3' 5 mM; D2O 99%

sample_2: 5'-D(*AP*GP*GP*AP*TP*CP*CP*TP*TP*TP*UP*GP*GP*AP*TP*CP*CP*T)-3' 5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.0; pressure: 1 atm; temperature: 305 K

sample_cond_2: pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
E-COSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

VNMR v6.1 - data analysis, data collection

DISCOVER v97 - structure calculation

UXNMR v3.0 - data collection

FELIX v97 - data analysis, data processing

NMR spectrometers:

  • Bruker AMX 500 MHz

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