BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4550

Title: NMR structure of the palindromic DNA decamer d(GCGTTAACGC)2

Authors: Smith, J.; Tsui, V.; Chazin, W.; Case, D.

Citation: Smith, J.; Tsui, V.; Chazin, W.; Case, D.. "NMR Structure of the Palindromic DNA Decamer d(GCGTTAACGC)2"  The BMRB entry is the only known published source for the data..

Assembly members:
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3', polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3': GCGTTAACGC

Data sets:
Data typeCount
1H chemical shifts83
coupling constants18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1strand 11
2strand 21

Entities:

Entity 1, strand 1 10 residues - Formula weight is not available

1   DGDCDGDTDTDADADCDGDC

Samples:

sample_1: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 99.9%

sample_2: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 95%

sample_cond_1: ionic strength: 70 mM; pH: 7; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablesample_cond_1
E-COSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

FELIX v95 - processing

NAB v2.1 - structure calculation

AMBER v5.0 - refinement, iterative matrix relaxation

NMR spectrometers:

  • Bruker AMX 500 MHz

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