BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4555

Title: 31P NMR analysis of the DNA conformation induced by protein binding:SRY-DNA complexes

Authors: Castagne, Claire; Murphy, Elizabeth; Gronenborn, Angela; Delepierre, Muriel

Citation: Castagne, Clairen; Murphy, Elizabeth; Gronenborn, Angela; Delepierre, Muriel. "31P NMR Analysis of the DNA Conformation Induced by Protein Binding SRY/DNA Complexes"  Eur. J. Biochem. 267, 1223-1229 (2000).

Assembly members:
SRY 14mer strand 1, polymer, 14 residues, Formula weight is not available
SRY 14mer strand 2, polymer, 14 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: na na

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
SRY 14mer strand 1: CCTGCACAAACACC
SRY 14mer strand 2: GGTGTTTGTGCAGG

Data sets:
Data typeCount
31P chemical shifts26

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
114mer strand 11
214mer strand 22

Entities:

Entity 1, 14mer strand 1 14 residues - Formula weight is not available

1   DCDCDTDGDCDADCDADADA
2   DCDADCDC

Entity 2, 14mer strand 2 14 residues - Formula weight is not available

1   DGDGDTDGDTDTDTDGDTDG
2   DCDADGDG

Samples:

SRY-14mer: SRY 14mer strand 1 1 mM; SRY 14mer strand 2 1 mM

cond1: pH: 7; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-1H COSYSRY-14mernot availablecond1
1H-1H TOCSYSRY-14mernot availablecond1
1H-1H NOESYSRY-14mernot availablecond1
1H-31P HSQC-TOCSYSRY-14mernot availablecond1
1H-31P HCOSYINVSRY-14mernot availablecond1
31P-31P NOESY ExchnageSRY-14mernot availablecond1

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz

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