BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4566

Title: Assignment of 1H,13C and 15N signals of Bovine Adrenodoxin

Authors: Weiss, Roland; Brachais, Laurent; Loehr, Frank; Hartleib, Judith; Bernhardt, Rita; Rueterjans, Heinz

Citation: Weiss, Roland; Brachais, Laurent; Lohr, Frank; Hartleib, Judith; Bernhardt, Rita; Rueterjans, Heinz. "Letter to the Editor: Assignment of 1H, 13C and 15N signals of bovine adrenodoxin"  J. Biomol. NMR 17, 355-356 (2000).

Assembly members:
bovine adrenodoxin, polymer, 128 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
bovine adrenodoxin: SSSEDKITVHFINRDGETLT TKGKIGDSLLDVVVQNNLDI DGFGACEGTLACSTCHLIFE QHIFEKLEAITDEENDMLDL AYGLTDRSRLGCQICLTKAM DNMTVRVPDAVSDARESIDM GMNSSKIE

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts576
13C chemical shifts415
15N chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bovine adrenodoxin1
22[FeS]2

Entities:

Entity 1, bovine adrenodoxin 128 residues - Formula weight is not available

1   SERSERSERGLUASPLYSILETHRVALHIS
2   PHEILEASNARGASPGLYGLUTHRLEUTHR
3   THRLYSGLYLYSILEGLYASPSERLEULEU
4   ASPVALVALVALGLNASNASNLEUASPILE
5   ASPGLYPHEGLYALACYSGLUGLYTHRLEU
6   ALACYSSERTHRCYSHISLEUILEPHEGLU
7   GLNHISILEPHEGLULYSLEUGLUALAILE
8   THRASPGLUGLUASNASPMETLEUASPLEU
9   ALATYRGLYLEUTHRASPARGSERARGLEU
10   GLYCYSGLNILECYSLEUTHRLYSALAMET
11   ASPASNMETTHRVALARGVALPROASPALA
12   VALSERASPALAARGGLUSERILEASPMET
13   GLYMETASNSERSERLYSILEGLU

Entity 2, 2[FeS] - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: bovine adrenodoxin, [U-100% 13C; U-100% 15N], 1.0 – 4.0 mM; D2O 5%

sample_2: bovine adrenodoxin, [U-100% 15N], 1.0 – 4.0 mM; D2O 5%

cond_1: pH: 7.4; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D-TOCSY-(15N,1H)HSQCnot availablenot availablecond_1
3D-NOESY-(15N,1H)HSQCnot availablenot availablecond_1
3D-HNHBnot availablenot availablecond_1
HNCAnot availablenot availablecond_1
HN(CO)CAnot availablenot availablecond_1
H(N)CA,COnot availablenot availablecond_1
HNCACBnot availablenot availablecond_1
HCACOnot availablenot availablecond_1
HNCOnot availablenot availablecond_1
(HCA)CO(CA)NHnot availablenot availablecond_1
HBHA(CO)NHnot availablenot availablecond_1
HCCH-TOCSYnot availablenot availablecond_1
HCCC(O)NHnot availablenot availablecond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX Avance 600 MHz

Related Database Links:

PDB
DBJ BAA00362 BAA00363
GB AAA30357 AAA30358 AAB21264 AAI09850 ABQ13041
REF NP_851354 XP_004329468 XP_005889885 XP_006072637 XP_010861227
SP P00257
TPG DAA22428