BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4568

Title: Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin

Authors: Eliezer, David; Chung, John; Dyson, Jane; Wright, Peter

Citation: Eliezer, David; Chung, John; Dyson, Jane; Wright, Peter. "Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin"  Biochemistry ., .-..

Assembly members:
myoglobin, polymer, 153 residues, Formula weight is not available

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
myoglobin: VLSEGEWQLVLHVWAKVEAD VAGHGQDILIRLFKSHPETL EKFDRFKHLKTEAEMKASED LKKHGVTVLTALGAILKKKG HHEAELKPLAQSHATKHKIP IKYLEFISEAIIHVLHSRHP GDFGADAQGAMNKALELFRK DIAAKYKELGYQG

Data sets:
Data typeCount
1H chemical shifts286
13C chemical shifts429
15N chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoMb1

Entities:

Entity 1, apoMb 153 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSHISGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHISPRO
13   GLYASPPHEGLYALAASPALAGLNGLYALA
14   METASNLYSALALEUGLULEUPHEARGLYS
15   ASPILEALAALALYSTYRLYSGLULEUGLY
16   TYRGLNGLY

Samples:

sample_1: myoglobin, [U-95% 13C; U-90% 15N; U-80% 2H], 0.3 mM; sodium acetate, [U-90% 2H], 10 mM; ethanol, [U-90% 2H], 10 % by volume

ex-cond_1: pH: 4.1; temperature: 323 K; ionic strength: 0.005 M

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availableex-cond_1
H(CA)CO(CA)NHsample_1not availableex-cond_1
HNCAsample_1not availableex-cond_1
HN(CO)CAsample_1not availableex-cond_1
HN(CA)CBsample_1not availableex-cond_1
CBCA(CO)NHsample_1not availableex-cond_1
1H-15N HSQC-NOESY-HSQCsample_1not availableex-cond_1

Software:

FELIX v4.2 - data processing

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4676 4695
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185