BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4570

Title: NMR Study of Sso7d Mutant (F31A) Minimized Average Structure

Authors: CONSONNI, R.; SANTOMO, L.; ZETTA, L.

Citation: Consonni, R.; Santomo, L.; Fusi, P.; Tortora, P.; Zetta, L.. "A Single-Point Mutation in the Extreme Heat- and Pressure-Resistant Sso7d From Sulfolobus Solfataricus Leads to a Major Rearrangement of the Hydrophobic Core"  Biochemistry 38, 12709-12717 (1999).

Assembly members:
SSO7D, polymer, 62 residues, 6958 Da.

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI

Entity Sequences (FASTA):
SSO7D: ATVKFKYKGEEKQVDISKIK KVWRVGKMISATYDEGGGKT GRGAVSEKDAPKELLQMLEK QK

Data sets:
Data typeCount
1H chemical shifts303

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso7d, F31A mutant1

Entities:

Entity 1, Sso7d, F31A mutant 62 residues - 6958 Da.

1   ALATHRVALLYSPHELYSTYRLYSGLYGLU
2   GLULYSGLNVALASPILESERLYSILELYS
3   LYSVALTRPARGVALGLYLYSMETILESER
4   ALATHRTYRASPGLUGLYGLYGLYLYSTHR
5   GLYARGGLYALAVALSERGLULYSASPALA
6   PROLYSGLULEULEUGLNMETLEUGLULYS
7   GLNLYS

Samples:

sample_1: SSO7D 2.0 mM

sample_cond_1: pD: 4.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
GE-TOCSYnot availablenot availablenot available
GE-NOESYnot availablenot availablenot available

Software:

FELIX v970, Molecular Simulations Inc. - resonance assignments

NMR spectrometers:

  • BRUKER DMX 500 MHz

Related Database Links:

PDB
DBJ BAA28274
GB AAB25172 AAK42090 AAK42212 ACP37540 ACP45250
PRF 1908252A
REF WP_009992021
SP P61990 P61991