BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4579

Title: Sequence-specific 1H, 15N and 13C resonance assignments of the EEA1 FYVE domain.

Authors: Kutateladze, Tatiana; Overduin, Michael

Citation: Kutateladze, Tatiana; Overduin, Michael. "Letter to the Editor: Sequence-specific 1H, 15N and 13C resonance assignments of the EEA1 FYVE domain"  J. Biomol. NMR 17, 89-90 (2000).

Assembly members:
Early Endosome autoantigen 1, polymer, 86 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Early Endosome autoantigen 1: AVQELGRENQSLQIKHTQAL NRKWAEDNEVQNCMACGKGF SVTVRRHHCRQCGNIFCAEC SAKNALTPSSKKPVRVCDAC FNDLQG

Data sets:
Data typeCount
1H chemical shifts575
13C chemical shifts366
15N chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FYVE1
2Zinc 12
3Zinc 22

Entities:

Entity 1, FYVE 86 residues - Formula weight is not available

1   ALAVALGLNGLULEUGLYARGGLUASNGLN
2   SERLEUGLNILELYSHISTHRGLNALALEU
3   ASNARGLYSTRPALAGLUASPASNGLUVAL
4   GLNASNCYSMETALACYSGLYLYSGLYPHE
5   SERVALTHRVALARGARGHISHISCYSARG
6   GLNCYSGLYASNILEPHECYSALAGLUCYS
7   SERALALYSASNALALEUTHRPROSERSER
8   LYSLYSPROVALARGVALCYSASPALACYS
9   PHEASNASPLEUGLNGLY

Entity 2, Zinc 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Early Endosome autoantigen 1 1.0 mM

sample_2: Early Endosome autoantigen 1, [U-15N], 1.0 mM

sample_3: Early Endosome autoantigen 1, [U-15N; U-13C], 1.0 mM

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availableEx-cond_1
2D COSYnot availablenot availableEx-cond_1
3D 1H-15N DIPSI-HSQCnot availablenot availableEx-cond_1
3D 1H-13C HCCH TOCSYnot availablenot availableEx-cond_1
2D 1H-15N HSQCnot availablenot availableEx-cond_1
2D 1H-13C HSQCnot availablenot availableEx-cond_1
2D HBCBCGCDHD TOCSYnot availablenot availableEx-cond_1
2D HBCBCGCDCEHE TOCSYnot availablenot availableEx-cond_1
3D HNCACBnot availablenot availableEx-cond_1
3D CBCA(CO)NHnot availablenot availableEx-cond_1
3D HNHAnot availablenot availableEx-cond_1
3D HNCOnot availablenot availableEx-cond_1
3D HCC TOCSYnot availablenot availableEx-cond_1
3D CCC TOCSYnot availablenot availableEx-cond_1
3D 1H-15N NOESYnot availablenot availableEx-cond_1
3D 1H-13C NOESYnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
EMBL CAA55632
GB AAA79121 AAI56546 AAI72504 EAW97471 EDM16847
REF NP_001101556 NP_003557 XP_001104577 XP_001510589 XP_001926390
SP Q15075