BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4581

Title: Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k

Authors: Maler, L.; Blankenship, J.; Rance, M.; Chazin, W.

Citation: Maler, L.; Blankenship, J.; Rance, M.; Chazin, W.. "Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k"  Nat. Struct. Biol. 7, 245-250 (2000).

Assembly members:
CALBINDIN D9K, polymer, 75 residues, Formula weight is not available

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CALBINDIN D9K: KSPEELKGIFEKYAAKEGDP NQLSKEELKLLLQTEFPSLL KGMSTLDELFEELDKAGDGE VSFEEFQVLVKKISQ

Data sets:
Data typeCount
1H chemical shifts480
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CALBINDIN D9K1

Entities:

Entity 1, CALBINDIN D9K 75 residues - Formula weight is not available

1   LYSSERPROGLUGLULEULYSGLYILEPHE
2   GLULYSTYRALAALALYSGLUGLYASPPRO
3   ASNGLNLEUSERLYSGLUGLULEULYSLEU
4   LEULEUGLNTHRGLUPHEPROSERLEULEU
5   LYSGLYMETSERTHRLEUASPGLULEUPHE
6   GLUGLULEUASPLYSALAGLYASPGLYGLU
7   VALSERPHEGLUGLUPHEGLNVALLEUVAL
8   LYSLYSILESERGLN

Samples:

sample_1: CALBINDIN D9K 4 mM

sample_2: CALBINDIN D9K, [U-15N], 4 mM

sample_cond_1: pH: 6.0; temperature: 300 K; ionic strength: 0 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
3D 15N-SEPARATED NOESYnot availablenot availablesample_cond_1
HSQC-Jnot availablenot availablesample_cond_1

Software:

FELIX v97.0 - resonance assignments, bookkeeping

DIANA v2.8 - structure solution

AMBER v4.1 - refinement

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz
  • Varian Inova 600 MHz

Related Database Links:

BMRB 15594 16340 16758 247 325 326 327
PDB
GB AAA30420 AAA72542 AAI18481
PRF 0707237A:PDB=3ICB
REF NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP P02633
TPG DAA12577