BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4583

Title: The C-terminal Domain of the RNA Polymerase Alpha Subunit from Thermus Thermophilus   PubMed: 10821859

Authors: Wada, T.; Yamazaki, T.; Kyogoku, Y.

Citation: Wada, T.; Yamazaki, T.; Kyogoku, Y.. "The structure and the characteristic DNA binding property of the C-terminal domain of the RNA polymerase alpha subunit from Thermus thermophilus"  J. Biol. Chem. 275, 16057-16063 (2000).

Assembly members:
RNA POLYMERASE ALPHA SUBUNIT, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RNA POLYMERASE ALPHA SUBUNIT: AAPEEAKEPEAPPEQEEELD LPLEELGLSTRVLHSLKEEG IESVRALLALNLKDLKNIPG IGERSLEEIKEALEKKGFTL KE

Data sets:
Data typeCount
1H chemical shifts480
13C chemical shifts251
15N chemical shifts83
coupling constants69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA POLYMERASE ALPHA SUBUNIT1

Entities:

Entity 1, RNA POLYMERASE ALPHA SUBUNIT 82 residues - Formula weight is not available

1   ALAALAPROGLUGLUALALYSGLUPROGLU
2   ALAPROPROGLUGLNGLUGLUGLULEUASP
3   LEUPROLEUGLUGLULEUGLYLEUSERTHR
4   ARGVALLEUHISSERLEULYSGLUGLUGLY
5   ILEGLUSERVALARGALALEULEUALALEU
6   ASNLEULYSASPLEULYSASNILEPROGLY
7   ILEGLYGLUARGSERLEUGLUGLUILELYS
8   GLUALALEUGLULYSLYSGLYPHETHRLEU
9   LYSGLU

Samples:

sample_1: RNA POLYMERASE ALPHA SUBUNIT, [U-13C; U-15N], 2 mM; phosphate 20 mM; KCl 30 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0; temperature: 310 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions

Software:

NMRPIPE - data collection

X-PLOR v3.1 - data processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAA75549 BAA88567 BAD71487
GB AAS81642 AEG34077 AFH38290 EIA38525
PIR JE0397
REF WP_008633361 WP_011173698 WP_014629067 YP_144930
SP Q5SHR6 Q72I32 Q9Z9H6