BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4584

Title: Solution Structure of the DNA-binding Domain of TraM   PubMed: 11258958

Authors: Stockner, T.; Plugariu, C.; Koraimann, G.; Hoegenauer, G.; Bermel, W.; Prytulla, S.; Sterk, H.

Citation: Stockner, T.; Plugariu, C.; Koraimann, G.; Hoegenauer, G.; Bermel, W.; Prytulla, S.; Sterk, H.. "Solution Structure of the DNA-binding Domain of TraM"  Biochemistry 40, 3370-3377 (2001).

Assembly members:
R1 PLASMID TRAM PROTEIN, polymer, 55 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
R1 PLASMID TRAM PROTEIN: AKVQAYVSDEIVYKINKIVE RRRAEGAKSTDVSFSSISTM LLELGLRVYEAQMER

Data sets:
Data typeCount
1H chemical shifts399
15N chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R1 PLASMID TRAM PROTEIN1

Entities:

Entity 1, R1 PLASMID TRAM PROTEIN 55 residues - Formula weight is not available

1   ALALYSVALGLNALATYRVALSERASPGLU
2   ILEVALTYRLYSILEASNLYSILEVALGLU
3   ARGARGARGALAGLUGLYALALYSSERTHR
4   ASPVALSERPHESERSERILESERTHRMET
5   LEULEUGLULEUGLYLEUARGVALTYRGLU
6   ALAGLNMETGLUARG

Samples:

sample_1: R1 PLASMID TRAM PROTEIN 4 mg/ml; phosphate buffer 50 mM; H2O 95%; D2O 5%

sample_2: R1 PLASMID TRAM PROTEIN, [U-15N], 1.3 mg/ml; phosphate buffer 50 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 4.0; temperature: 300 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D-15N-NOESY-HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

XWINNMR v2.5 - collection

NMRPipe - processing

ANSIG v3.3 - data analysis

X-PLOR v3.851 - refinement

MOLMOL v2.6 - data analysis

VNMR v5.2 - collection

NMR spectrometers:

  • Varian UnityInova 600 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI57888
EMBL CAQ87481 CAU95771 CBG27852 CBJ04497 CCA64277
GB AAA92656 ABE10679 ACQ42006 ACU68816 ADL14025
PRF 1110195A
REF WP_001151555 WP_001151556 WP_001151557 WP_001151559 WP_001151560
SP P07294