BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4587

Title: NMR STRUCTURAL DETERMINATION OF VISCOTOXIN A3 FROM VISCUM ALBUM L.   PubMed: 10947973

Authors: Ugolini, R.; Fogolari, F.; Schaller, G.; Urech, K.; Giannattasio, M.; Ragona, L.; Molinari, H.

Citation: Romagnoli, Silvia; Ugolini, Raffaella; Fogolari, Federico; Schaller, Gerhard; Urech, Konrad; Giannattasio, Matteo; Ragona, Laura; Molinari, Henriette. "NMR structural determination of viscotoxin A3 from Viscum album L"  Biochem. J. 350, 569-577 (2000).

Assembly members:
Viscotoxin A3, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: European mistletoe   Taxonomy ID: 3972   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Viscum album

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Viscotoxin A3: KSCCPNTTGRNIYNACRLTG APRPTCAKLSGCKIISGSTC PSDYPK

Data sets:
Data typeCount
1H chemical shifts308

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Viscotoxin A31

Entities:

Entity 1, Viscotoxin A3 46 residues - Formula weight is not available

1   LYSSERCYSCYSPROASNTHRTHRGLYARG
2   ASNILETYRASNALACYSARGLEUTHRGLY
3   ALAPROARGPROTHRCYSALALYSLEUSER
4   GLYCYSLYSILEILESERGLYSERTHRCYS
5   PROSERASPTYRPROLYS

Samples:

sample_1: Viscotoxin A31.0 – 2.0 mM; H3PO4/NaOH aqueous buffer 50 mM; H2O 90%; D2O 10%

sample_2: Viscotoxin A31.0 – 2.0 mM; H3PO4/NaOH aqueous buffer 50 mM; D2O 100%

sample_cond_1: ionic strength: 50 mM; pH: 3.6; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available

Software:

DYANA v1.4 - solution structure calculation

DISCOVER v98 - refinement

xwinnmr v1.2 - collection

XEASY v1.2 - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAB29759
PIR VTVAA3
PRF 2007441A
SP P01538