BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4589

Title: NMR Solution Structure of the Last Unknown Module of the Cellulosomal Scaffoldin Protein CIPC of Clostridum cellulolyticum

Authors: Mosbah, A.; Belaich, A.; Bornet, O.; Belaich, J.; Henrissat, B.; Darbon, H.

Citation: Mosbah, A.; Belaich, A.; Bornet, O.; Belaich, J.; Henrissat, B.; Darbon, H.. "NMR Solution Structure of the Last Unknown Module of the Cellulosomal Scaffoldin Protein CIPC of Clostridum cellulolyticum"  J. Mol. Biol. 304, 201-217 (2000).

Assembly members:
scaffoldin protein, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Clostridium cellulolyticum   Taxonomy ID: 1521   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium cellulolyticum

Experimental source:   Production method: .

Entity Sequences (FASTA):
scaffoldin protein: MQDPTINPTSISAKAGSFAD TKITLTPNGNTFNGISELQS SQYTKGTNEVTLLASYLNTL PENTTKTLTFDFGVGTKNPK LTITVLPKDIPGLE

Data sets:
Data typeCount
15N chemical shifts86
1H chemical shifts525
coupling constants77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1scaffoldin protein1

Entities:

Entity 1, scaffoldin protein 94 residues - Formula weight is not available

1   METGLNASPPROTHRILEASNPROTHRSER
2   ILESERALALYSALAGLYSERPHEALAASP
3   THRLYSILETHRLEUTHRPROASNGLYASN
4   THRPHEASNGLYILESERGLULEUGLNSER
5   SERGLNTYRTHRLYSGLYTHRASNGLUVAL
6   THRLEULEUALASERTYRLEUASNTHRLEU
7   PROGLUASNTHRTHRLYSTHRLEUTHRPHE
8   ASPPHEGLYVALGLYTHRLYSASNPROLYS
9   LEUTHRILETHRVALLEUPROLYSASPILE
10   PROGLYLEUGLU

Samples:

sample_1: scaffoldin protein, [U-15N], 25 mM; sodium acetate buffer 20 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
3D 15N-separated_NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
2D 15N HSQCsample_1not availablesample_cond_1
2D 15N HSQC_NOESYsample_1not availablesample_cond_1
2D 15N HSQC_TOCSYsample_1not availablesample_cond_1

Software:

xwinnmr v2.1 - processing

XEASY v1.2 - data analysis

DIANA v2.8 - structure solution

CNS v1.0 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

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