BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4590

Title: Solution structure of the human chemokine Eotaxin-2

Authors: Mayer, K.; Stone, M.

Citation: Mayer, K.; Stone, M.. "NMR Solution Structure and Receptor Peptide Binding of the CC-CHemokine Eotaxin-2 "  Biochemistry 39, 8382-8395 (2000).

Assembly members:
EOTAXIN-2, polymer, 73 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
EOTAXIN-2: VVIPSPCCMFFVSKRIPENR VVSYQLSSRSTCLKAGVIFT TKKGQQSCGDPKQEWVQRYM KNLDAKQKKASPR

Data sets:
Data typeCount
1H chemical shifts461
13C chemical shifts252
15N chemical shifts76
coupling constants51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EOTAXIN-21

Entities:

Entity 1, EOTAXIN-2 73 residues - Formula weight is not available

1   VALVALILEPROSERPROCYSCYSMETPHE
2   PHEVALSERLYSARGILEPROGLUASNARG
3   VALVALSERTYRGLNLEUSERSERARGSER
4   THRCYSLEULYSALAGLYVALILEPHETHR
5   THRLYSLYSGLYGLNGLNSERCYSGLYASP
6   PROLYSGLNGLUTRPVALGLNARGTYRMET
7   LYSASNLEUASPALALYSGLNLYSLYSALA
8   SERPROARG

Samples:

sample_1: EOTAXIN-2, [U-15N; U-13C], 1 mM; NaAc 20 mM; H2O 90%; D2O 10%

sample_2: EOTAXIN-2, [U-13C], 1 mM; NaAc 20 mM; H2O 90%; D2O 10%

sample_3: EOTAXIN-2, [U-15N; U-13C], 1 mM; NaAc 20 mM; D2O 100%

sample_4: EOTAXIN-2, [U-10% 13C], 1 mM; NaAc 20 mM; D2O 100%

sample_cond_1: pH: 4.1; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions

Software:

VNMR - collection

FELIX v98 - processing

X-PLOR v98 - structure solution

NMR spectrometers:

  • Varian Unity INOVA 500 MHz

Related Database Links:

PDB
DBJ BAG35149 BAJ21120
EMBL CAA11383
GB AAB51135 AAD15410 AAH69072 AAH69391 ADQ32923
REF NP_002982 XP_001155246 XP_003808972 XP_004045658 XP_009451649
SP O00175