BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4592

Title: Solution structure of the syndecan-4 whole cytoplasmic domain in the presence of phosphatidylinositol 4,5-bisphosphate

Authors: Shin, J.; Oh, E.; Lee, D.; Couchman, J.; Lee, W.

Citation: Lee, D.; Oh, E.; Woods, A.; Couchman, J.; Lee, W.. "Solution Structure of a Syndecan-4 Cytoplasmic Domain and Its Interaction with Phosphatidylinositol 4,5-Bisphosphate "  J. Biol. Chem. 273, 13022-13029 (1998).

Assembly members:
Syndecan 4, polymer, 28 residues, Formula weight is not available
PT5, non-polymer, 1047.088 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: not reported

Entity Sequences (FASTA):
Syndecan 4: RMKKKDEGSYDLGKKPIYKK APTNEFYA

Data sets:
Data typeCount
1H chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Syndecan 4 monomer 11
2Syndecan 4 monomer 21
3PIP22

Entities:

Entity 1, Syndecan 4 monomer 1 28 residues - Formula weight is not available

1   ARGMETLYSLYSLYSASPGLUGLYSERTYR
2   ASPLEUGLYLYSLYSPROILETYRLYSLYS
3   ALAPROTHRASNGLUPHETYRALA

Entity 2, PIP2 - C47 H85 O19 P3 - 1047.088 Da.

1   PT5

Samples:

sample_1: Syndecan 42 – 4 mM; PIP20.5 – 1 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_2: Syndecan 42 – 4 mM; PIP20.5 – 1 mM; phosphate buffer 50 mM; D2O 100%

sample_cond_1: pH: 7.4; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
DQF COSYnot availablenot availablesample_cond_1

Software:

XWINNMR v2.5 - data collection, data processing

Sparky v3.6 - data analysis

X-PLOR v3.851 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 4591
PDB
DBJ BAA02550 BAA19613 BAA22135 BAA22136 BAD96415
EMBL CAA47406 CAG46842 CAG46871 CAH91121
GB AAA16479 AAA73167 AAB26725 AAH02312 AAH05679
REF NP_001007870 NP_001127378 NP_001162499 NP_001244363 NP_001269761
SP O35988 P31431 P34901 P49416 Q5RAT9
TPG DAA22957