BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4593

Title: Rous sarcoma virus capsid protein: C-terminal domain

Authors: Kingston, Richard; Fitzon-Ostendorp, Tanja; Eisenmesser, Elan; Schatz, Gisela; Vogt, Volker; Post, Carol; Rossmann, Michael

Citation: Kingston, Richard; Fitzon-Ostendorp, Tanja; Eisenmesser, Elan; Schatz, Gisela; Vogt, Volker; Post, Carol; Rossmann, Michael. "Structure and Self-association of the Rous Sarcoma Virus Capsid Protein"  Structure Fold Des. 8, 617-628 (2000).

Assembly members:
RSV_CA, polymer, 96 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: Virus   Kingdom: not available   Genus/species: Avian type C retroviruses Rous sarcoma virus - Prague C strain

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RSV_CA: MDIMQGPSESFVDFANRLIK AVEGSDLPPSARAPVIIDCF RQKSQPDIQQLIRTAPSTLT TPGEIIKYVLDRQKTAPLTD QGIAAAMSSAIQPLIM

Data sets:
Data typeCount
1H chemical shifts554
13C chemical shifts252
15N chemical shifts80
coupling constants122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RSV CA1

Entities:

Entity 1, RSV CA 96 residues - Formula weight is not available

1   METASPILEMETGLNGLYPROSERGLUSER
2   PHEVALASPPHEALAASNARGLEUILELYS
3   ALAVALGLUGLYSERASPLEUPROPROSER
4   ALAARGALAPROVALILEILEASPCYSPHE
5   ARGGLNLYSSERGLNPROASPILEGLNGLN
6   LEUILEARGTHRALAPROSERTHRLEUTHR
7   THRPROGLYGLUILEILELYSTYRVALLEU
8   ASPARGGLNLYSTHRALAPROLEUTHRASP
9   GLNGLYILEALAALAALAMETSERSERALA
10   ILEGLNPROLEUILEMET

Samples:

sample_1: RSV_CA, [U-15N], 1.5 mM; Sodium phosphate 50 mM; NaCl 50 mM; EDTA 1 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_2: RSV_CA, [U-15N; U-13C], 1.5 mM; Sodium phosphate 50 mM; NaCl 50 mM; EDTA 1 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.9; temperature: 298 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available

Software:

NMRPipe v1.6 - data processing

ANSIG v3.3 - data analysis

CNS v1.0 - refinement

NMR spectrometers:

  • Varian UnityPlus 600 MHz

Related Database Links:

PDB
EMBL CAA24512 CAA36153 CAA48534
GB AAA66157 AAB59932 AAB59933 AAC82560 AAC82561
REF NP_056886 NP_056887 NP_955609
SP P03322 P03354