BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4599

Title: High-Resolution Solution Structure of the 18 kDa Substrate-Binding Domain of the Mammalian Chaperone Protein Hsc70

Authors: Morshauser, R.; Hu, W.; Wang, H.; Pang, Y.; Flynn, G.; Zuiderweg, E.

Citation: Morshauser, R.; Hu, W.; Wang, H.; Pang, Y.; Flynn, G.; Zuiderweg, E.. "High-Resolution Solution Structure of the 18 kDa Substrate-Binding Domain of the Mammalian Chaperone Protein Hsc70 "  J. Mol. Biol. 289, 1387-1403 (1999).

Assembly members:
heat shock cognate 70 kd protein 1, polymer, 161 residues, Formula weight is not available

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
heat shock cognate 70 kd protein 1: SENVQDLLLLDVTPLSLGIE TAGGVMTVLIKRNTTIPTKQ TQTFTTYSDNQPGVLIQVYE GERAMTKDNNLLGKFELTGI PPAPRGVPQIEVTFDIDANG ILNVSAVDKSTGKENKITIT NDKGRLSKEDIERMVQEAEK YKAEDEKQRDKVSSKNSLEH H

Data sets:
Data typeCount
1H chemical shifts979
13C chemical shifts657
15N chemical shifts153

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HSC701

Entities:

Entity 1, HSC70 161 residues - Formula weight is not available

1   SERGLUASNVALGLNASPLEULEULEULEU
2   ASPVALTHRPROLEUSERLEUGLYILEGLU
3   THRALAGLYGLYVALMETTHRVALLEUILE
4   LYSARGASNTHRTHRILEPROTHRLYSGLN
5   THRGLNTHRPHETHRTHRTYRSERASPASN
6   GLNPROGLYVALLEUILEGLNVALTYRGLU
7   GLYGLUARGALAMETTHRLYSASPASNASN
8   LEULEUGLYLYSPHEGLULEUTHRGLYILE
9   PROPROALAPROARGGLYVALPROGLNILE
10   GLUVALTHRPHEASPILEASPALAASNGLY
11   ILELEUASNVALSERALAVALASPLYSSER
12   THRGLYLYSGLUASNLYSILETHRILETHR
13   ASNASPLYSGLYARGLEUSERLYSGLUASP
14   ILEGLUARGMETVALGLNGLUALAGLULYS
15   TYRLYSALAGLUASPGLULYSGLNARGASP
16   LYSVALSERSERLYSASNSERLEUGLUHIS
17   HIS

Samples:

sample_1: heat shock cognate 70 kd protein 1, [U-15N], 1.5 mM; sodium phosphate 50 mM; D2O 5%; H2O 95%

sample_2: heat shock cognate 70 kd protein 1, [U-15N; U-13C], 1.5 mM; sodium phosphate 50 mM; D2O 5%; H2O 95%

sample_3: heat shock cognate 70 kd protein 1, [U-15N], 1.5 mM; sodium phosphate 50 mM; D2O 100%

sample_4: heat shock cognate 70 kd protein 1, [U-15N; U-13C], 1.5 mM; sodium phosphate 50 mM; D2O 100%

sample_cond_1: pH: 7.0; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablesample_cond_1
HN(CA)HAnot availablenot availablesample_cond_1
HN(CO)CAnot availablenot availablesample_cond_1
HA(CACO)NHnot availablenot availablesample_cond_1
CP H(C)CCH-TOCSYnot availablenot availablesample_cond_1
CP (H)CCH-TOCSYnot availablenot availablesample_cond_1
CP(H)C(CCACO)NH-TOCSYnot availablenot availablesample_cond_1
15N RESOLVED NOESY-HSQCnot availablenot availablesample_cond_1
13C RESOLVED NOESY-HMQCnot availablenot availablesample_cond_1
4D 13C-13C RESOLVED HMQC-NOESYnot availablenot availablesample_cond_1
3D 13C-13N RESOLVED HMQC-NOESY-HSQCnot availablenot availablesample_cond_1
3D 13C-13C RESOLVED HMQC-NOESY-HSQC-HSQCnot availablenot availablesample_cond_1

Software:

DISCOVER - refinement

FELIX - structure calculation

MSI INSIGHT vINSIGHT - structure calculation

MSI DISCOVER vDISCOVER - structure calculation

NMR spectrometers:

  • BRUKER DMX 750 MHz

Related Database Links:

BMRB 4497
PDB
DBJ BAB69718 BAC29016 BAC36065 BAD12572 BAD96505
EMBL CAA06233 CAA37422 CAA37823 CAA49670 CAA68265
GB AAA36991 AAA37869 AAA41354 AAB18391 AAC52836
PIR S31716
REF NP_001075247 NP_001079632 NP_001080068 NP_001091238 NP_001125783
SP A2Q0Z1 O73885 P11142 P19120 P19378
TPG DAA22199 DAA34064