BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4695

Title: Effect of the single mutation His 64->Phe on the stability and folding of apomyoglobin   PubMed: 10985768

Authors: Garcia, Carlos; Cavagnero, Silvia; Nishimura, Chiaki; Dyson, H.; Wright, Peter

Citation: Garcia, Carlos; Nishimura, Chiaki; Cavagnero, Silvia; Dyson, H.; Wright, Peter. "Changes in the apomyoglobin folding pathway caused by mutation of the distal histidine residue"  Biochemistry 39, 11227-11237 (2000).

Assembly members:
myoglobin, polymer, 153 residues, Formula weight is not available
HEA, non-polymer, 852.837 Da.

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
myoglobin: VLSEGEWQLVLHVWAKVEAD VAGHGQDILIRLFKSHPETL EKFDRFKHLKTEAEMKASED LKKFGVTVLTALGAILKKKG HHEAELKPLAQSHATKHKIP IKYLEFISEAIIHVLHSRHP GDFGADAQGAMNKALELFRK DIAAKYKELGYQG

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts147
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myoglobin H64F mutant1

Entities:

Entity 1, myoglobin H64F mutant 153 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSPHEGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHISPRO
13   GLYASPPHEGLYALAASPALAGLNGLYALA
14   METASNLYSALALEUGLULEUPHEARGLYS
15   ASPILEALAALALYSTYRLYSGLULEUGLY
16   TYRGLNGLY

Samples:

sample_1: myoglobin, [U-95% 13C; U-90% 15N], 1 mM

ex-cond_1: pH: 5.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableex-cond_1
CBCA(CO)NHsample_1not availableex-cond_1

Software:

FELIX v97 - process and plot spectra

NMRView - process and plot

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

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PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185